Abstract
A new wheat–Leymus mollis (2n = 4x = 28, NsNsXmXm) disomic addition line M11003-3-1-12-10 (M10) was developed and selected from the BC1F5 progeny of a cross between wheat cv. 7182 and octoploid Tritileymus M47 (2n = 8x = 56, AABBDDNsNs). Cytological observations showed that M10 contained 44 chromosomes and formed 22 bivalents at meiotic metaphase I. The sequential fluorescence in situ hybridization–genomic in situ hybridization (FISH–GISH) analysis using the oligonucleotide Oligo-pSc119.2, Oligo-pTa535 and L. mollis genomic DNA as probes revealed that M10 carried a pair of L. mollis chromosomes and a whole set of 42 wheat chromosomes. Two EST–STS markers and six PLUG (PCR-based Landmark Unique Gene) markers confirmed that the added L. mollis chromosomes in M10 belonged to homoeologous group 7 and were designated as Lm#7Ns, so M10 was deduced to be a wheat–L. mollis Lm#7Ns disomic addition line. Field disease evaluation demonstrated that M10 showed a high level of resistance to stripe rust at the adult stage and the resistance could be ultimately traced to the L. mollis. Consequently, the introduced Lm#7Ns chromosomes were responsible for the stripe rust resistance. The novel disomic addition line M10 could be exploited as an important genetic resource for wheat resistance breeding.
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References
An DG, Ma PT, Zheng Q et al (2019) Development and molecular cytogenetic identification of a new wheat–Rye 4R chromosome disomic addition line with resistances to powdery mildew, stripe rust and sharp eyespot. Theor Appl Genet 132(1):257–272. https://doi.org/10.1007/s00122-018-3214-3
Anamthawat-Jónsson K (1995) Wide-hybrids between wheat and lymegrass breeding and agricultural potential. Icel Agric Sci 9:101–113
Anamthawat-Jónsson K, Bodvarsdottir SK et al (1997) Wide hybridization between wheat (Triticum L.) and lymegrass (Leymus Hochst.). Euphytica 93:293–300. https://doi.org/10.1023/A:1002965322262
Bao YG, Wang J, He F et al (2012) Molecular cytogenetic identification of a wheat (Triticum aestivum)–American dune grass (Leymus mollis) translocation line resistant to stripe rust. Genet Mol Res 11:3198–3206. https://doi.org/10.4238/2012.May.22.2
Chen XM (2005) Epidemiology and control of stripe rust on wheat (Puccinia striiformis f. sp. tritici) on wheat. Can J Plant Pathol 27:314–337. https://doi.org/10.1080/07060660509507230
Chen XM (2007) Challenges and solutions for stripe rust control in the United States. Aust J Agric Res 58:648–655. https://doi.org/10.1071/AR07045
Chen SY, Fu J, Gao LZ (1985) The hybridization between Triticum aestivum and Leymus mollis. Acta Bot Boreali-Occidentalia Sin 4:260–266
Chen GL, Zheng Q, Bao YG et al (2012) Molecular cytogenetic identification of a novel dwarf wheat line with introgressed Thinopyrum ponticum chromatin. J Biosci 37:149–155. https://doi.org/10.1007/s12038-011-9175-1
Deng CL, Bai LL, Fu SL et al (2013) Microdissection and chromosome painting of the alien chromosome in an addition line of wheat–Thinopyrum intermedium. PLoS ONE 8(8):e72564. https://doi.org/10.1371/journal.pone.0072564
Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
Du WL, Wang J, Wang LM et al (2013) Development and characterization of a Psathyrostachys huashanica Keng 7Ns chromosome addition line with leaf rust resistance. PLoS ONE 8:e70879. https://doi.org/10.1371/journal.pone.0070879
Fan X, Sha LN, Yang RW et al (2009) Phylogeny and evolutionary history of Leymus (Triticeae; Poaceae) based on a single-copy nuclear gene encoding plastid acetyl-CoA carboxylase. BMC Evol Biol 9(1):247. https://doi.org/10.1186/1471-2148-9-247
Fatih AMB (1983) Analysis of the breeding potential of wheat–Agropyron and wheat–Elymus derivatives, 1 agronomic and quality characteristics. Hereditas 98:287–295
Fu SL, Yang MY, Ren ZL et al (2014) Abnormal mitosis induced by wheat–rye 1R monosomic addition lines. Genome 57(1):21–28. https://doi.org/10.1139/gen-2013-0115
Ge RC, Zhang JY, Zhao BC et al (2006) Identification of the alien chromosome in a salt-resistant wheat–Leymus multicaulis disomic addition line. Sci Agric Sin 39(1):193–198. https://doi.org/10.3321/j.issn:0578-1752.2006.01.028.
Habora ME, Eltayeb AE, Tsujimoto H et al (2012) Identification of osmotic stress-responsive genes from Leymus mollis, a wild relative of wheat (Triticum aestivum L.). Breed Sci 62(1):78–86. https://doi.org/10.1270/jsbbs.62.78
Habora ME, Eltayeb AE, Oka M et al (2013) Cloning of allene oxide cyclase gene from Leymus mollis and analysis of its expression in wheat–Leymus chromosome addition lines. Breed Sci 63(1):68–76. https://doi.org/10.1270/jsbbs.63.68
Han FP, Liu B, Fedak G (2004) Genomic constitution and variation in five partial amphiploids of wheat–Thinopyrum intermedium as revealed by GISH, multicolor GISH and seed storage protein analysis. Theor Appl Genet 109:1070–1076. https://doi.org/10.1007/s00122-004-1720-y
Han FP, Lamb JC, Birchler JA (2006) High frequency of centromere inactivation resulting in stable dicentric chromosomes of maize. Proc Natl Acad Sci USA 103(9):3238–3243. https://doi.org/10.1073/pnas.0509650103
Hu LJ, Li GR, Zeng ZX et al (2010) Molecular cytogenetic identification of a new wheat–Thinopyrum substitution line with stripe rust resistance. Euphytica 177:169–177. https://doi.org/10.1007/s10681-010-0216-x
Hu LJ, Li GR, Zhan HX et al (2012) New St-chromosome specific molecular markers for identifying wheat–Thinopyrum intermedium derivative lines. J Genet 91:e67–e74. https://doi.org/10.1007/s12041-012-0158-2
Ishikawa G, Yonemaru J, Saito M et al (2007) PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes. BMC Genom 8:135. https://doi.org/10.1186/1471-2164-8-135
Ishikawa G, Nakamura T, Ashida T et al (2009) Localization of anchor loci representing five hundred annotated rice genes to wheat chromosomes using PLUG markers. Theor Appl Genet 118:499–514. https://doi.org/10.1007/s00122-008-0916-y
Jiang SM, Yin WB, Hu J et al (2009) Isolation of expressed sequences from a specific chromosome of Thinopyrum intermedium infected by BYDV. Genome 52:68–76. https://doi.org/10.1139/g08-108
Kang HY, Wang Y, Fedak G et al (2011) Introgression of chromosome 3Ns from Psathyrostachys huashanica into wheat specifying resistance to stripe rust. PLoS ONE 6:e21802. https://doi.org/10.1371/journal.pone.0021802
Kishii M, Wang RRC, Tsujimoto H (2003) Characteristics and behaviour of the chromosomes of Leymus mollis and L. racemosus (Triticeae, Poaceae) during mitosis and meiosis. Chromosome Res 11:741–748. https://doi.org/10.1023/b:chro.0000005774.00726.71
Kong F, Wang HY, Cao AZ et al (2008) Characterization of T. aestivum–H. californicum chromosome addition lines Da2H and Ma5H. J Genet Genom 35:673–678
Lei MP, Li GR, Zhang SF et al (2011) Molecular cytogenetic characterization of a new wheat Secale africanum 2Ra (2D) substitution line for resistance to stripe rust. J Genet Genom 90:283–287. https://doi.org/10.1007/s12041-011-0081-y
McGuire PE, Dvorak J (1981) High salt-tolerance potential in wheatgrass. Crop Sci 21:702–705
Merker A (1992) The Triticeae in cereal breeding. Hereditas 116:277–280. https://doi.org/10.1111/j.1601-5223.1992.tb00154.x
Mujeeb-Kazi A, Bernard M, Bekele GT et al (1983) Incorporation of alien genetic information from Elymus giganteus into Triticum aestivum. In: Sakamoto S (ed) Proceedings of the 6th international wheat genetics symposium. Plant germplasm Institute, Kyoto, Japan, pp 223–231
Qi LL, Pumphrey MO, Friebe B et al (2011) A novel Robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat. Theor Appl Genet 123:159–167. https://doi.org/10.1007/s00122-011-1574-z
Saari EE, Prescott JM (1975) A scale for appraising the foliar intensity of wheat diseases. Plant Dis Rep 59:377–380
Said M, Cabrera A (2009) A physical map of chromosome 4Hch from H. chilense containing SSR, STS and EST–SSR molecular markers. Euphytica 167:253–259. https://doi.org/10.1007/s10681-009-9895-6
Sanguinetti CJ, Neto ED, Simpson AJG (1994) RAPD silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques 17(5):914–921. https://doi.org/10.1002/bmc.1130080616
Sigurbjornsson B (1960) Studies on the Icelandic Elymus. PhD thesis, Cornell University, Ithaca, New York
Tang ZX, Yang ZJ, Fu SL (2014) Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis. J Appl Genet 55:313–318. https://doi.org/10.1007/s13353-014-0215-z
Wang RRC (2011) Agropyron and psathyrostachys. In: Kole C (ed) Wild crop relatives genomic and breeding resources, cereals. Springer, Berlin, pp 77–108
Wang XP, Fu J, Zhang XQ et al (2000) Molecular cytogenetic study on genome constitutions of octoploid Tritileymus. Acta Bot Sin 42:582–586. https://doi.org/10.1023/A:1002769218797
Wang L, Yuan JH, Bie TD et al (2009) Cytogenetic and molecular identification of three Triticum aestivum–Leymus racemosus translocation addition lines. J Genet Genom 36(6):379–385. https://doi.org/10.1016/s1673-8527(08)60127-7
Wang D, Zhuang LF, Sun L et al (2010) Allocation of a powdery mildew resistance locus to the chromosome arm 6RL of Secale cereale L. cv. ‘Jingzhouheimai’. Euphytica 176:157–166. https://doi.org/10.1007/s10681-010-0199-7
Wang J, Chen XH, Du WL et al (2012) Morphological and molecular cytogenetic characterization of partial octoploid Tritileymus. Genet Resour Crop Evol 60:1453–1462. https://doi.org/10.1007/s10722-012-9933-4
Wang YJ, Quan W, Peng NN et al (2016) Molecular cytogenetic identification of a wheat–Aegilops geniculata Roth 7Mg disomic addition line with powdery mildew resistance. Mol Breed 36:40–52. https://doi.org/10.1007/s11032-016-0463-1
Wu J, Yang XM, Wang H et al (2006) The introgression of chromosome 6P specifying for increased numbers of florets and kernels from Agropyron cristatum into wheat. Theor Appl Genet 114:13–20. https://doi.org/10.1007/s00122-006-0405-0
Yan L, You Q, Mao Z et al (2002) Studied on Triticum aestivum–Leymus multicaulis disomic addition line (line24) resistance to PAV and GAV strains of barley yellow dwarf virus. Acta Phys Sin 32(3):247–251
Yang XF, Wang CY, Chen CH et al (2014) Chromosome constitution and origin analysis in three derivatives of Triticum aestivum–Leymus mollis by molecular cytogenetic identification. Genome 57:583–591. https://doi.org/10.1139/gen-2014-0161
Yang XF, Wang CY, Li X et al (2015) Development and molecular cytogenetic identification of a novel wheat–Leymus mollis Lm#7Ns (7D) disomic substitution line with stripe rust resistance. PLoS ONE 10(10):e0140227. https://doi.org/10.1371/journal.pone.0140227
Yang XF, Li X, Wang CY et al (2017) Isolation and molecular cytogenetic characterization of a wheat–Leymus mollis double monosomic addition line and its progenies with resistance to stripe rust. Genome 60(12):1029–1036. https://doi.org/10.1139/gen-2017-0078
Yaniv E, Raats D, Ronin Y et al (2015) Evaluation of marker-assisted selection for the stripe rust resistance gene Yr15, introgressed from wild emmer wheat. Mol Breed 35:1–12. https://doi.org/10.1007/s11032-015-0238-0
Zhang P, Friebe B, Gill BS et al (2007) Cytogenetics in the age of molecular genetics. Aust J Agric Res 58:498–506. https://doi.org/10.1071/AR07054
Zhao JX, Du WL, Wu J et al (2013) Development and identification of a wheat–Leymus mollis multiple alien substitution line. Euphytica 190:45–52. https://doi.org/10.1007/s10681-012-0772-3
Zhou RN, Shi R, Jiang SM et al (2008) Rapid EST isolation from chromosome 1R of rye. BMC Plant Biol 8(3):576. https://doi.org/10.1186/1471-2229-8-28
Acknowledgements
This work was supported by grants from the National Natural Science Foundation of China (Grant No. 31701416), Scientific research plan projects of Shaanxi Education Department (Grant No. 20JK0629), Talent project of Weinan Normal University (Grant No. 18ZRRC14), Zhongying Tang Breeding Funding Project of USA at the Northwest A&F University in China and the innovation project of science and technology of Shaanxi province of China (Grant No. 2015KTZDNY01-01-02).
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Yang, X.F., Wang, C.Y., Chen, C.H. et al. Development and characterization of a wheat–Leymus mollis Lm#7Ns disomic addition line with resistance to stripe rust. CEREAL RESEARCH COMMUNICATIONS 48, 467–476 (2020). https://doi.org/10.1007/s42976-020-00058-5
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DOI: https://doi.org/10.1007/s42976-020-00058-5