A procedure was developed for introducing tritium into the side chain of the abscisic acid (ABA) molecule. The reaction was carried out in dioxane in the presence of the Lindlar catalyst. The yield of the labeled product was 70% and the molar radioactivity was 44 Ci/mol. The tritium-labeled abscisic acid analogue was found to be a growth substrate for soil bacteria that incorporate a radioactive label into cellular metabolites. The absorption of the label by bacteria from the side chain of abscisic acid is more than an order of magnitude higher than that for labeling in the cyclohexene moiety. The results indicate the existence of a previously unknown metabolism pathway of ABA in microorganisms.

中文翻译：

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环己烯或侧基中类似氚标记的脱落酸代谢的比较研究

开发了一种将氚引入脱落酸 (ABA) 分子侧链的程序。该反应在Lindlar催化剂存在下在二恶烷中进行。标记产物的产率为70%，摩尔放射性为44 Ci/mol。氚标记的脱落酸类似物被发现是土壤细菌的生长底物，这些细菌将放射性标记结合到细胞代谢物中。细菌对脱落酸侧链标记的吸收比环己烯部分的标记高一个数量级以上。结果表明微生物中存在一种以前未知的 ABA 代谢途径。