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Comparative Study of the Abscisic Acid Metabolism Using Analogue Tritium-Labeled in the Cyclohexene or Side Moiety

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Abstract

A procedure was developed for introducing tritium into the side chain of the abscisic acid (ABA) molecule. The reaction was carried out in dioxane in the presence of the Lindlar catalyst. The yield of the labeled product was 70% and the molar radioactivity was 44 Ci/mol. The tritium-labeled abscisic acid analogue was found to be a growth substrate for soil bacteria that incorporate a radioactive label into cellular metabolites. The absorption of the label by bacteria from the side chain of abscisic acid is more than an order of magnitude higher than that for labeling in the cyclohexene moiety. The results indicate the existence of a previously unknown metabolism pathway of ABA in microorganisms.

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Funding

The work was partly supported by the Russian Science Foundation, project no. 17–14–01363 and Fundamental Research Programs of the Presidium of the RAS “Innovative Research in Biomedicine” and “Postgenomic Technologies and Prospective Solutions in Biochemistry.”

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Authors and Affiliations

  1. Institute of Molecular Genetics, Russian Academy of Sciences, 123182, Moscow, Russia

    V. P. Shevchenko, I. Yu. Nagaev, K. V. Shevchenko &  N. F. Myasoedov

  2. All-Russia Research Institute for Agricultural Microbiology, Russian Academy of Sciences, 196608, St. Petersburg, Russia

    A. I. Shaposhnikov, A. A. Belimov & O. S. Yuzikhin

  3. All-Russian Institute of Plant Protection, 196608, St. Petersburg, Russia

    O. S. Yuzikhin

  4. Kazan Institute of Biochemistry and Biophysics, Structural Branch of the Federal Research Center “Kazan Scientific Center of the Russian Academy of Sciences”, 420111, Kazan, Russia

    T. T. Ismailov, N. E. Gogoleva & Yu. V. Gogolev

  5. Kazan (Volga Region) Federal University, 420008, Kazan, Russia

    T. S. Ermekkaliev, N. E. Gogoleva & Yu. V. Gogolev

Authors
  1. V. P. Shevchenko
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  2. I. Yu. Nagaev
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  3. A. I. Shaposhnikov
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  4. K. V. Shevchenko
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  5. A. A. Belimov
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  6. O. S. Yuzikhin
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  7. T. T. Ismailov
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  8. T. S. Ermekkaliev
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  9. N. E. Gogoleva
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  10. Yu. V. Gogolev
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  11. N. F. Myasoedov
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Corresponding author

Correspondence to I. Yu. Nagaev.

Additional information

Translated by Z. Svitanko

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Shevchenko, V.P., Nagaev, I.Y., Shaposhnikov, A.I. et al. Comparative Study of the Abscisic Acid Metabolism Using Analogue Tritium-Labeled in the Cyclohexene or Side Moiety. Dokl Chem 491, 41–44 (2020). https://doi.org/10.1134/S0012500820030015

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  • DOI: https://doi.org/10.1134/S0012500820030015

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