An ultrasensitive electrochemiluminescence biosensor was established based on the Zn-MOF/GO nanocomposite. Ag(I)-embedded DNA complexes were used as a signal amplification reagent. In this work, 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin (TCPP) and Zn2+ were integrated into a porphyrin paddlewheel framework (Zn-MOF) by a hydrothermal method. The synthesized Zn-MOF material has electrochemiluminescence property, and the luminescence intensity is improved after being composited with graphene oxide (GO). Based on the composite material, we constructed an ultrasensitive ECL biosensor for the p53 antibody detection. The composite material acted as an admirable substrate and then loaded plenty of p53 antigens to recognize the target (p53 antibody) accurately. Because of the bridging effect of streptavidin and biotin-conjugated goat anti-rabbit IgG (bio-ab2), the rich-C DNA with positive correlation with the target was modified on the electrode and then captured the co-reactant accelerator Ag+ to amplify the signal. Therefore, the ECL biosensor response increases with increasing p53 antibody concentration. In the range 0.1 fg/mL–0.01 ng/mL, the response signal of the biosensor has a good linear relationship with the p53 antibody concentration. The detection limit is 0.03 fg/mL (S/N = 3). Impressively, the biosensor not only featured high sensitivity, good stability, and excellent specificity for the detection of p53 antibody, but also provides a new way for early detection of cancer. Graphical abstract Schematic representation of the electrochemiluminescence sensor based on a Zn-MOF/GO nanocomposite, which can be applied to the determination of p53 antibody. Schematic representation of the electrochemiluminescence sensor based on a Zn-MOF/GO nanocomposite, which can be applied to the determination of p53 antibody.

中文翻译：

---

基于Zn-MOF/GO纳米复合材料和Ag+-DNA扩增的p53抗体电化学发光生物传感器

建立了基于Zn-MOF/GO纳米复合材料的超灵敏电化学发光生物传感器。Ag(I) 嵌入的 DNA 复合物用作信号放大试剂。在这项工作中，通过水热法将 5,10,15,20-四（4-羧基苯基）卟啉（TCPP）和 Zn2+ 集成到卟啉桨轮骨架（Zn-MOF）中。合成的Zn-MOF材料具有电化学发光特性，与氧化石墨烯（GO）复合后发光强度得到提高。基于该复合材料，我们构建了用于 p53 抗体检测的超灵敏 ECL 生物传感器。复合材料充当了令人钦佩的基材，然后加载了大量 p53 抗原以准确识别目标（p53 抗体）。由于链霉亲和素与生物素偶联的山羊抗兔IgG（bio-ab2）的桥连作用，在电极上修饰与靶标呈正相关的富C DNA，然后捕获共反应加速剂Ag+以放大信号。因此，ECL 生物传感器响应随着 p53 抗体浓度的增加而增加。在 0.1 fg/mL–0.01 ng/mL 范围内，生物传感器的响应信号与 p53 抗体浓度具有良好的线性关系。检测限为 0.03 fg/mL (S/N = 3)。令人印象深刻的是，该生物传感器不仅对p53抗体的检测具有灵敏度高、稳定性好、特异性好等特点，而且为癌症的早期检测提供了新的途径。基于Zn-MOF/GO纳米复合材料的电化学发光传感器的图形摘要示意图，可用于p53抗体的测定。基于 Zn-MOF/GO 纳米复合材料的电化学发光传感器的示意图，可用于检测 p53 抗体。