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An electrochemiluminescence biosensor for p53 antibody based on Zn-MOF/GO nanocomposite and Ag+-DNA amplification

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Abstract

An ultrasensitive electrochemiluminescence biosensor was established based on the Zn-MOF/GO nanocomposite. Ag(I)-embedded DNA complexes were used as a signal amplification reagent. In this work, 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin (TCPP) and Zn2+ were integrated into a porphyrin paddlewheel framework (Zn-MOF) by a hydrothermal method. The synthesized Zn-MOF material has electrochemiluminescence property, and the luminescence intensity is improved after being composited with graphene oxide (GO). Based on the composite material, we constructed an ultrasensitive ECL biosensor for the p53 antibody detection. The composite material acted as an admirable substrate and then loaded plenty of p53 antigens to recognize the target (p53 antibody) accurately. Because of the bridging effect of streptavidin and biotin-conjugated goat anti-rabbit IgG (bio-ab2), the rich-C DNA with positive correlation with the target was modified on the electrode and then captured the co-reactant accelerator Ag+ to amplify the signal. Therefore, the ECL biosensor response increases with increasing p53 antibody concentration. In the range 0.1 fg/mL–0.01 ng/mL, the response signal of the biosensor has a good linear relationship with the p53 antibody concentration. The detection limit is 0.03 fg/mL (S/N = 3). Impressively, the biosensor not only featured high sensitivity, good stability, and excellent specificity for the detection of p53 antibody, but also provides a new way for early detection of cancer.

Schematic representation of the electrochemiluminescence sensor based on a Zn-MOF/GO nanocomposite, which can be applied to the determination of p53 antibody.

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Funding

This work was supported by the National Natural Science Foundation of China (21976001), Natural Science Foundation of Anhui Province (1808085QB53), and open fund for Discipline Construction of Institute of Physical Science and Information Technology.

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Authors and Affiliations

  1. Key Laboratory of Structure and Functional Regulation of Hybrid Materials (Ministry of Education), Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, School of Chemistry & Chemical Engineering, Anhui University, Hefei, Anhui, 230601, People’s Republic of China

    Yu-ping Wei, Yi-wen Zhang, Jing-Shuai Chen, Chang-jie Mao & Bao-Kang Jin

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  1. Yu-ping Wei
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  2. Yi-wen Zhang
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  3. Jing-Shuai Chen
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  4. Chang-jie Mao
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Correspondence to Jing-Shuai Chen or Chang-jie Mao.

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The protocol of this study was approved by the medical ethics committee of The First Affiliated Hospital of Anhui Medical University. Human Serum Samples were also provided by The First Affiliated Hospital of Anhui Medical University. The blood samples (2 mL) of the participants were collected after informed written consent was provided by the subjects.

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Wei, Yp., Zhang, Yw., Chen, JS. et al. An electrochemiluminescence biosensor for p53 antibody based on Zn-MOF/GO nanocomposite and Ag+-DNA amplification. Microchim Acta 187, 455 (2020). https://doi.org/10.1007/s00604-020-04425-1

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