The development of a gold nanoparticle aptamer assay is persued for rapid and sensitive determination of histamine in foodstuffs, which could be deployed for on-site use. The assay is based on a histamine-specific aptamer and gold nanoparticles and the salt-induced aggregation of the particles in the presence of histamine indicated by the color change from red to blue. Gold nanoparticle size, salt type, and concentration as well as aptamer concentration were optimized, and using optimum conditions, a limit of detection of 8 nM (~ 0.05 mg/kg) was obtained. Finally, the aptamer AuNP assay was applied to the determination of histamine in quality control fish samples. The histamine levels of these samples had previously been determined using HPLC and commercial ELISA kits by numerous independent laboratories and a good correlation was obtained. The developed AuNP assay is rapid, sensitive, and reproducible. Graphical abstract Graphical abstract

中文翻译：

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金纳米粒子适体测定法测定食品中的组胺

金纳米颗粒适体测定法的开发用于快速、灵敏地测定食品中的组胺，可用于现场使用。该测定基于组胺特异性适体和金纳米粒子，以及在组胺存在下盐诱导的粒子聚集，由红色到蓝色的颜色变化表示。优化了金纳米颗粒的大小、盐类型和浓度以及适体浓度，并使用最佳条件获得了 8 nM (~ 0.05 mg/kg) 的检测限。最后，将适体 AuNP 测定应用于质量控制鱼样品中组胺的测定。这些样品的组胺水平先前已由许多独立实验室使用 HPLC 和商业 ELISA 试剂盒测定，并获得了良好的相关性。开发的 AuNP 检测快速、灵敏且可重复。图形摘要图形摘要