Abstract

The contents of conjugated covalently polyamines (CC-PAs) and conjugated non-covalently polyamines (CNC-PAs) in embryo tonoplast, and the vacuole conformation were investigated using the embryos of developing maize (Zea mays L.) grains of two cultivars differing in drought-tolerance as experiment materials. The results indicated that after drought stress treatment for seven days, relative water content of embryo (RWCE) and relative increase rate of embryo dry weight (RIREDW) of the drought-sensitive cultivar ‘Xund-an no. 22’ decreased more significantly than those of the drought-tolerant ‘Zhengdan No. 958’. Furthermore, the changes in vacuole conformation of ‘Xundan no. 22’ were more marked. Meanwhile, the increases of the contents of CNC-spermidine (CNC-Spd) and CC-putrescine (CC-Put) in embryo tonoplast from ‘Zhengdan no. 958’ were more obvious than those from ‘Xundan no. 22’. Treatment with exogenous Spd not only alleviated the injury of drought stress to ‘Xundan no. 22’, but also enhanced the increases of CNC-Spd content and inhibited the changes in the vacuole conformation of this cultivar. The treatments of ‘Zhengdan no. 958’ with two inhibitors, methylglyoxyl-bis (guanylhydrazone) and phenanthrolin, significantly inhibited the drought stress-induced increases of CNC-Spd and CC-Put contents of the cultivar, respectively. Meanwhile, the treatments significantly aggravated the injury of drought stress to ‘Zhengdan no. 958’ and the changes in the vacuole conformation of this cultivar. All the results mentioned above suggest that the CNC-Spd and CC-Put in embryo tonoplast isolated from developing grains could enhance the tolerance of maize to drought stress by maintaining the vacuole conformation.

中文翻译：

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与胚液泡膜共轭的多胺可通过维持液泡形态来增强玉米对干旱胁迫的耐受性

摘要

使用显影玉米的胚胎（胚胎液泡膜，和液泡构共价缀合的多胺（CC-PAS）和共轭的或非共价的多胺（CNC-PAS）的内容进行了调查玉蜀黍L.）两个品种的耐旱性不同的籽粒作为实验材料。结果表明，干旱胁迫处理7天后，干旱敏感品种'Xund-an no。2'的胚相对含水量（RWCE）和胚干重相对增加率（RIREDW）增加。22'比耐旱的'单旦958号'下降得更明显。此外，'迅旦号'的液泡构象的变化。22'更明显。同时，郑单号胚胎液泡膜中CNC-亚精胺（CNC-Spd）和CC-鸟氨酸（CC-Put）的含量增加。958”比“寻单号”中的那些更明显。22'。外源Spd处理不仅减轻了干旱胁迫对'旬丹No。22'，而且还增加了CNC-Spd含量的增加，并抑制了该品种液泡构象的变化。正丹一号的治疗方法 带有两种抑制剂的958'甲基乙醛双（胍基hydr）和菲咯啉分别显着抑制干旱胁迫诱导的品种CNC-Spd和CC-Put含量的增加。同时，处理显着加剧了干旱胁迫对“正单号”的伤害。958'和该品种液泡构象的变化。以上所有结果表明，从发育中的谷物中分离出的胚液膜中的CNC-Spd和CC-Put可以通过维持液泡的构象来增强玉米对干旱胁迫的耐受性。甲基乙醛双（胍基hydr）和菲咯啉分别显着抑制干旱胁迫诱导的品种CNC-Spd和CC-Put含量增加。同时，处理显着加剧了干旱胁迫对“正单号”的伤害。958'和该品种液泡构象的变化。以上所有结果表明，从发育中的谷物中分离出的胚液膜中的CNC-Spd和CC-Put可以通过维持液泡的构象来增强玉米对干旱胁迫的耐受性。甲基乙醛双（胍基hydr）和菲咯啉分别显着抑制干旱胁迫诱导的品种CNC-Spd和CC-Put含量增加。同时，处理显着加剧了干旱胁迫对“正单号”的伤害。958'和该品种液泡构象的变化。以上所有结果表明，从发育中的谷物中分离出的胚液膜中的CNC-Spd和CC-Put可以通过维持液泡的构象来增强玉米对干旱胁迫的耐受性。958'和该品种液泡构象的变化。以上所有结果表明，从发育中的谷物中分离出的胚液膜中的CNC-Spd和CC-Put可以通过维持液泡的构象来增强玉米对干旱胁迫的耐受性。958'和该品种液泡构象的变化。以上所有结果表明，从发育中的谷物中分离出的胚液膜中的CNC-Spd和CC-Put可以通过维持液泡的构象来增强玉米对干旱胁迫的耐受性。