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Polyamines Conjugated to Embryo Tonoplast Enhance the Tolerance of Maize to Drought Stress by Maintaining the Vacuole Conformation

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Abstract

The contents of conjugated covalently polyamines (CC-PAs) and conjugated non-covalently polyamines (CNC-PAs) in embryo tonoplast, and the vacuole conformation were investigated using the embryos of developing maize (Zea mays L.) grains of two cultivars differing in drought-tolerance as experiment materials. The results indicated that after drought stress treatment for seven days, relative water content of embryo (RWCE) and relative increase rate of embryo dry weight (RIREDW) of the drought-sensitive cultivar ‘Xund-an no. 22’ decreased more significantly than those of the drought-tolerant ‘Zhengdan No. 958’. Furthermore, the changes in vacuole conformation of ‘Xundan no. 22’ were more marked. Meanwhile, the increases of the contents of CNC-spermidine (CNC-Spd) and CC-putrescine (CC-Put) in embryo tonoplast from ‘Zhengdan no. 958’ were more obvious than those from ‘Xundan no. 22’. Treatment with exogenous Spd not only alleviated the injury of drought stress to ‘Xundan no. 22’, but also enhanced the increases of CNC-Spd content and inhibited the changes in the vacuole conformation of this cultivar. The treatments of ‘Zhengdan no. 958’ with two inhibitors, methylglyoxyl-bis (guanylhydrazone) and phenanthrolin, significantly inhibited the drought stress-induced increases of CNC-Spd and CC-Put contents of the cultivar, respectively. Meanwhile, the treatments significantly aggravated the injury of drought stress to ‘Zhengdan no. 958’ and the changes in the vacuole conformation of this cultivar. All the results mentioned above suggest that the CNC-Spd and CC-Put in embryo tonoplast isolated from developing grains could enhance the tolerance of maize to drought stress by maintaining the vacuole conformation.

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Funding

The research is supported by National Natural Science Foundation of China (project no. 31271627), Science and Technology Program of Henan Province (project no. 192102110135) and Key Scientific Research Projects of Higher School in Henan Province (project no. 20A210032).

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Authors and Affiliations

  1. College of Life Science and Agronomy/Henan Key Laboratory of Crop Molecular Breeding and Bioreactor, Zhoukou Normal University, 466001, Zhoukou Henan, China

    H. Y. Du, J. M. Yu, H. P. Liu & R. Kurtenbach

  2. College of Biological Science, China Agricultural University, 100193, Beijing, China

    G. T. Liu

  3. Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou China University, 225000, Yangzhou Jiangsu, China

    D. X. Liu

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  1. H. Y. Du
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  2. G. T. Liu
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  4. J. M. Yu
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  5. H. P. Liu
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  6. R. Kurtenbach
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Correspondence to H. P. Liu.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants as objects of research.

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Abbreviations: CC—conjugated covalently; CNC—conjugated non-covalently; MGBG—methylglyoxyl-bis-guanylhydrazone; o-Phen—phenanthrolin; PAs—polyamines; PCA—perchloric acid; PVP—polyvinyl pyrrolidone; RIREDW—relative increase rate of embryo dry weight; RWCE—relative water content of embryo; SAMDC—S-adenosylmethionine decarboxylase.

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Du, H.Y., Liu, G.T., Liu, D.X. et al. Polyamines Conjugated to Embryo Tonoplast Enhance the Tolerance of Maize to Drought Stress by Maintaining the Vacuole Conformation. Russ J Plant Physiol 67, 724–732 (2020). https://doi.org/10.1134/S1021443720040044

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