Abstract

This research was carried out to determine effectiveness of nitric oxide (NO) on some physiological and biochemical reactions, and water use efficiency (WUE) of chard (Beta vulgaris L. var. cicla) under well-watered and drought conditions. NO solutions were prepared with four doses (0, 50, 100, 150 and 200 µM) of sodium nitroprusside as a nitric oxide donor and exogenously applied on only seeds (s) or together with seed and foliar (sf) of chard under different levels of drought according to 100 (control), 67 and 33% of the water required to reach field capacity. NO mitigated the negative effects of drought stress on fresh and dry weights of leaf and root, chlorophyll content, gas exchange parameters and electrical leakage in all doses, especially for doses of 100 and 150 µM for both s and sf applications. Higher antioxidant enzyme content was observed with the application of NO especially under well-watered conditions. Mild drought conditions caused a desirable increase on WUE of the chard.

中文翻译：

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干旱胁迫下一氧化氮暴露的唐莴苣生理指标和一些抗氧化酶的测定

摘要

进行这项研究以确定一氧化氮（NO）对某些生理和生化反应的有效性以及唐莴苣（Beta vulgaris）的水分利用效率（WUE）L.var。cicla）在干旱和干旱的条件下。用四剂（0、50、100、150和200 µM）硝普钠作为一氧化氮供体制备NO溶液，并仅在种子上或与种子和叶面（sf）外生施于不同水平的硝普钠作为一氧化氮供体。根据100（对照）的干旱情况，达到田间生产能力所需的水量分别为67％和33％。在所有剂量下，NO均可减轻干旱胁迫对叶片和根的鲜重和干重，叶绿素含量，气体交换参数和漏电的负面影响，特别是对于s和sf应用而言，剂量分别为100和150 µM。尤其是在水分充足的条件下，使用NO可以观察到更高的抗氧化酶含量。温和的干旱条件导致唐莴苣的水分利用效率增加。