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Determination of Physiological Indices and Some Antioxidant Enzymes of Chard Exposed to Nitric Oxide under Drought Stress

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Abstract

This research was carried out to determine effectiveness of nitric oxide (NO) on some physiological and biochemical reactions, and water use efficiency (WUE) of chard (Beta vulgaris L. var. cicla) under well-watered and drought conditions. NO solutions were prepared with four doses (0, 50, 100, 150 and 200 µM) of sodium nitroprusside as a nitric oxide donor and exogenously applied on only seeds (s) or together with seed and foliar (sf) of chard under different levels of drought according to 100 (control), 67 and 33% of the water required to reach field capacity. NO mitigated the negative effects of drought stress on fresh and dry weights of leaf and root, chlorophyll content, gas exchange parameters and electrical leakage in all doses, especially for doses of 100 and 150 µM for both s and sf applications. Higher antioxidant enzyme content was observed with the application of NO especially under well-watered conditions. Mild drought conditions caused a desirable increase on WUE of the chard.

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Funding

We are very grateful to the Scientific Research Projects Foundation, Atatürk University for its financial support (project no. BAP-169).

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Correspondence to S. Ors.

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This article does not contain any studies involving animals or human participants as objects of research. The authors declare that they have no conflict of interest.

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Abbreviations: CAT—catalase, Ci—concentration of intercellular CO2, EL—electrolyte leakage, ETa—actual evapotranspiration, gs–stomatal conductance, LCRV—leaf chlorophyll reading values, NO—nitric oxide, PAR—photosynthetically active radiation, Pn—photosynthetic rate, PMSF—phenylmethanesulphonylfluoride, POD—peroxidase, PVP—polyvinylpyrrolidone, RWC—relative water content, SOD—superoxide dismutase, Tr—transpiration rate, T—turgid weight, WUE—water use efficiency.

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Ekinci, M., Ors, S., Yildirim, E. et al. Determination of Physiological Indices and Some Antioxidant Enzymes of Chard Exposed to Nitric Oxide under Drought Stress. Russ J Plant Physiol 67, 740–749 (2020). https://doi.org/10.1134/S1021443720040056

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  • DOI: https://doi.org/10.1134/S1021443720040056

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