Fibromyalgia is a refractory syndrome characterized by chronic wayward pain and complex co-morbid psychological trepidation. The current treatments have a limited role and proper clinical benefits are far from satisfactory. Naturally occurring coumarins such as osthole are known to have analgesic and anti-inflammatory activities. Therefore, the current investigation was designed to explore the potential of natural coumarin esculetin (2.5, 5, and 10 mg/kg) in mitigating reserpine-induced fibromyalgia in Swiss albino mice. Esculetin is a 6,7 dihydroxy-coumarin obtained from various plant sources such as Aesculus hippocastanum L, Ceratostigma willmottianum, Citrus limonia, etc. Reserpine (0.5 mg/kg/day s.c.) treatment for first 3 days, significantly altered the behavior of mice as evidenced by reduced paw withdrawal threshold in pressure application measurement (PAM) test and electronic von-Frey (eVF) test, increased immobility time in forced swim test (FST), increased latency to reach the platform in Morris water maze (MWM) test and reduced number of square crossed in the open field test (OFT). These behavioral deficits with reserpine treatment were integrated with a reduced level of serotonin (5-HT), reduced glutathione (GSH), along with an increase in monoamine oxidase-A (MAO-A) activity, pro-inflammatory cytokines (IL-1β, TNF-α), thiobarbituric acid reactive substances (TBARS) and glutamate level. Esculetin (10 mg/kg/day i.p) treatment for 5 days, significantly abrogated reserpine induced behavioral and biochemical alterations. Whereas, no significant improvement was observed with lower doses of esculetin i.e. 2.5 and 5 mg/kg.

纤维肌痛是一种顽固性综合征，其特征是慢性任性疼痛和复杂的合并症心理震颤。当前的治疗作用有限，适当的临床益处远不能令人满意。天然存在的香豆素，例如osthole，具有镇痛和抗炎作用。因此，本研究旨在探索天然香豆素七叶皂甙（2.5、5和10 mg / kg）在缓解瑞士白化病小鼠中利血平诱导的纤维肌痛中的潜力。Esculetin是一种6,7二羟基香豆素，得自多种植物来源，例如七叶树七叶树，柳叶角柏，柑橘柠檬头三天利血平（0.5 mg / kg /天sc）处理显着改变了小鼠的行为，这可以通过压力应用测量（PAM）测试和电子冯-弗雷（eVF）测试中爪缩回阈值的降低来证明，在强迫游泳测试（FST）中增加了不动时间，在莫里斯水迷宫（MWM）测试中到达平台的等待时间增加，并且在野外测试（OFT）中减少了交叉的平方数。利血平治疗的这些行为缺陷与5-羟色胺（5-HT）水平降低，谷胱甘肽（GSH）降低，单胺氧化酶-A（MAO-A）活性增加，促炎细胞因子（IL-1β）整合在一起，TNF-α），硫代巴比妥酸反应性物质（TBARS）和谷氨酸水平。Esculetin（10 mg / kg / day ip）治疗5天，消除了利血平引起的行为和生化改变。然而，较低的七叶亭剂量（即2.5和5 mg / kg）未观察到明显改善。