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Protocatechuic acid supplement alleviates allergic airway inflammation by inhibiting the IL-4Rα-STAT6 and Jagged 1/Jagged2-Notch1/Notch2 pathways in allergic asthmatic mice.
Inflammation Research ( IF 4.8 ) Pub Date : 2020-07-15 , DOI: 10.1007/s00011-020-01379-1
Qin Li 1, 2, 3 , Yinfan Wu 1, 2, 4 , Xingyue Guo 1, 2, 4 , Ren Wang 5 , Yuheng Mao 1, 2, 3 , Yimin Zhao 1, 2, 3 , Jinchao Zou 1, 2, 3 , Chunwei Li 6, 7 , Yanqiu Chen 8 , Yan Yang 1, 2, 3
Affiliation  

Objective and design

To clarify the effects of dietary supplementation of protocatechuic acid (PCA) and in-depth mechanisms on allergic asthma in ovalbumin (OVA)-induced mice.

Materials

Female BALB/c mice were randomly divided into three groups (n = 10 in each group): control group, OVA-induced allergic asthma group, and OVA plus PCA group.

Treatment

Dietary supplementation of PCA was achieved by adding 50 mg/kg PCA to AIN 93G diet for 25 days.

Methods

Peripheral blood cells, pulmonary inflammatory cell infiltration, the levels of IL-4, IL-5, and IL-13 in bronchoalveolar lavage fluid (BALF), the mRNA levels of Th2-related genes in the lungs, and the protein expressions of the IL-4Rα–STAT6 and the Jagged1/Jagged2–Notch1/Notch2 signaling pathways were measured.

Results

Significantly reduced inflammatory cells infiltration and mucosal hypersecretion in the lung tissues, repaired levels of interleukin IL-4, IL-5, and IL-13 in the BALF, and decreased mRNA expression of IL-4, IL-5, and GATA3 were observed in OVA plus PCA group. Moreover, PCA treatment down-regulated the protein levels of IL-4Rα–STAT6 and Jagged1/Jagged2–Notch1/Notch2 signaling pathways.

Conclusions

Dietary supplement of PCA alleviated allergic asthma partly through suppressing the IL-4Rα–STAT6 and Jagged1/Jagged2–Notch1/Notch2 signaling pathways in mice. Our study provided the theoretic basis of PCA used as functional food in preventing allergic asthma.



中文翻译:

原儿茶酸补充剂通过抑制过敏性哮喘小鼠的 IL-4Rα-STAT6 和 Jagged 1/Jagged2-Notch1/Notch2 通路减轻过敏性气道炎症。

目标和设计

阐明膳食补充原儿茶酸 (PCA) 的影响以及对卵清蛋白 (OVA) 诱导小鼠过敏性哮喘的深入机制。

材料

将雌性 BALB/c 小鼠随机分为三组(每组n  = 10):对照组、OVA 诱发的过敏性哮喘组和 OVA 加 PCA 组。

治疗

PCA 的膳食补充是通过在 AIN 93G 饮食中添加 50 mg/kg PCA 25 天来实现的。

方法

外周血细胞、肺部炎症细胞浸润、支气管肺泡灌洗液(BALF)中 IL-4、IL-5、IL-13 水平,肺部 Th2 相关基因 mRNA 水平,以及测量了 IL-4Rα–STAT6 和 Jagged1/Jagged2–Notch1/Notch2 信号通路。

结果

肺组织炎症细胞浸润和黏膜高分泌显着减少,BALF中白细胞介素IL-4、IL-5和IL-13水平修复,IL-4、IL-5和GATA3 mRNA表达降低在 OVA 加 PCA 组中。此外,PCA 治疗下调 IL-4Rα–STAT6 和 Jagged1/Jagged2–Notch1/Notch2 信号通路的蛋白质水平。

结论

膳食补充剂 PCA 部分通过抑制小鼠的 IL-4Rα-STAT6 和 Jagged1/Jagged2-Notch1/Notch2 信号通路缓解过敏性哮喘。本研究为PCA作为功能性食品预防过敏性哮喘提供了理论依据。

更新日期:2020-07-16
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