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Exemplifying rhizobacteria for growth stimulation and disease suppression in finger millet
Journal of Plant Diseases and Protection ( IF 2 ) Pub Date : 2020-07-15 , DOI: 10.1007/s41348-020-00352-8
Poonam Kumari , R. S. Netam , Prafull Kumar

Of 111 rhizobacteria isolated from finger millet, 30 isolates exhibiting antagonism against Pyricularia grisea (22.22–44.44%) and Rhizoctonia solani (22.22–38.89%) were selected for functional characterization and in vitro interaction with pathogens. Appraisal of rhizobacteria for antimetabolites revealed HCN production by 3, ammonia production by all (0.80–4.39 µmol/ml) and H2S production by 5 isolates, while all produced siderophore (25.89–93.96%), 20 salicylic acid (3.12–54.46 µg/ml), 19 tested positive for glucanase, 17 for protease and 12 for amylase production. Culture filtrate of all the isolates inhibited growth of Rhizoctonia solani (23.33–81.11%) and Pyricularia grisea (19.35–53.22%). Role of thermostable metabolites in antagonism was observed with 21 isolates against Rhizoctonia solani (11.11–50.0%) and 24 against Pyricularia grisea (8.33–46.66%). Volatile antimetabolites of all the isolates retarded and inhibited growth of Pyricularia grisea (5.88–84.71%) and Rhizoctonia solani (5.5–83.33%), besides affecting mycelial quality and sclerotiation in Rhizoctonia solani. Metabolic extracts of 9 antagonists in ethyl acetate restricted the growth of Pyricularia grisea (8.88–20.80%) and of 10 inhibited Rhizoctonia solani (10.12–23.30%). Enlightening the involvement of bacterial growth regulators in plant growth stimulation, all isolates tested positive for IAA production (1.9–28.8 µg/ml in absence and 9.9–45.7 µg/ml in presence of tryptophan), 19 secreted ACC deaminase and 19 solubilized phosphate, while only 5 exhibited Zn-solubilization potential. Increase in plant height, root length, their fresh weight and chlorophyll content was recorded with seed priming under glasshouse condition.



中文翻译:

举例说明根瘤菌以促进小米的生长和抑制疾病

从小米中分离出的111种根瘤菌中,有30种表现出对稻瘟病菌Pyricularia grisea)(22.22–44.44%)和根瘤菌(Rhizoctonia solani)(22.22–38.89%)具有拮抗作用的菌株被选为功能鉴定和与病原体的体外相互作用。根瘤菌对抗代谢物的评估显示,HCN的产生率为3,全部产生的氨为(0.80–4.39 µmol / ml),5种分离物产生的H 2 S,而所有产生的铁载体(25.89–93.96%),20水杨酸(3.12–54.46) µg / ml),其中19例检测为葡聚糖酶阳性,17例检测为蛋白酶,12例检测为淀粉酶。所有分离株的培养滤液均抑制了状枯萎病菌Rhizoctonia solani)(23.33–81.11%)和稻瘟病菌的生长。(19.35–53.22%)。观察到热稳定代谢产物在拮抗作用中的作用,其中有21株针对状枯萎病菌(11.11–50.0%)和24株针对稻瘟病菌(8.33–46.66%)的菌株。所有的菌株挥发性代谢物延缓和抑制细菌的生长稻瘟病菌(5.88-84.71%)和水稻纹枯病菌(5.5-83.33%),除了影响菌丝体质量和sclerotiation纹枯病。9种拮抗剂在乙酸乙酯中的代谢物提取物限制了稻瘟病菌的生长(8.88–20.80%),而10种抑制作用的茄状枯萎病菌(10.12–23.30%)。为了启发细菌生长调节剂参与植物生长刺激,所有分离株的IAA产量均呈阳性(不存在时为1.9–28.8 µg / ml,在存在色氨酸时为9.9–45.7 µg / ml),19种分泌的ACC脱氨酶和19种溶解的磷酸盐,而只有5个具有Zn增溶潜能。在温室条件下,通过种子灌浆记录了株高,根长,鲜重和叶绿素含量的增加。

更新日期:2020-07-15
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