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Exemplifying rhizobacteria for growth stimulation and disease suppression in finger millet

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Abstract

Of 111 rhizobacteria isolated from finger millet, 30 isolates exhibiting antagonism against Pyricularia grisea (22.22–44.44%) and Rhizoctonia solani (22.22–38.89%) were selected for functional characterization and in vitro interaction with pathogens. Appraisal of rhizobacteria for antimetabolites revealed HCN production by 3, ammonia production by all (0.80–4.39 µmol/ml) and H2S production by 5 isolates, while all produced siderophore (25.89–93.96%), 20 salicylic acid (3.12–54.46 µg/ml), 19 tested positive for glucanase, 17 for protease and 12 for amylase production. Culture filtrate of all the isolates inhibited growth of Rhizoctonia solani (23.33–81.11%) and Pyricularia grisea (19.35–53.22%). Role of thermostable metabolites in antagonism was observed with 21 isolates against Rhizoctonia solani (11.11–50.0%) and 24 against Pyricularia grisea (8.33–46.66%). Volatile antimetabolites of all the isolates retarded and inhibited growth of Pyricularia grisea (5.88–84.71%) and Rhizoctonia solani (5.5–83.33%), besides affecting mycelial quality and sclerotiation in Rhizoctonia solani. Metabolic extracts of 9 antagonists in ethyl acetate restricted the growth of Pyricularia grisea (8.88–20.80%) and of 10 inhibited Rhizoctonia solani (10.12–23.30%). Enlightening the involvement of bacterial growth regulators in plant growth stimulation, all isolates tested positive for IAA production (1.9–28.8 µg/ml in absence and 9.9–45.7 µg/ml in presence of tryptophan), 19 secreted ACC deaminase and 19 solubilized phosphate, while only 5 exhibited Zn-solubilization potential. Increase in plant height, root length, their fresh weight and chlorophyll content was recorded with seed priming under glasshouse condition.

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Acknowledgements

Authors are thankful to Department of Science and Technology, Government of India, New Delhi, for possible funding under Knowledge Involvement in Research Advancement through Nurturing (KIRAN) program (Woman Scientist Scheme-A) which made this work a success.

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  1. S G College of Agriculture and Research Station, Jagdalpur, Indira Gandhi Agricultural University, Raipur, India

    Poonam Kumari, R. S. Netam & Prafull Kumar

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  1. Poonam Kumari
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  2. R. S. Netam
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Kumari, P., Netam, R.S. & Kumar, P. Exemplifying rhizobacteria for growth stimulation and disease suppression in finger millet. J Plant Dis Prot 127, 783–798 (2020). https://doi.org/10.1007/s41348-020-00352-8

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