A novel fluorescent probe, 2-allyl-6-((2-((2-hydroxy-5-nitrobenzylidene)amino)ethyl)amino)-1H-benzo[de]isoquinoline- 1,3(2H)-dione (ABID), based on naphthalimide-Schiff base, has been designed and synthesized for the monitoring of Cu²⁺ ions. In solution (DMSO/HEPES, 1:1, v/v, pH 7.4), ABID displayed fluorescence quenching towards Cu²⁺ ions over other important metal ions. A good linearity with a correlation coefficient (R²) of 0.99 validated that the ABID probe could be used to detect Cu²⁺ ions in 0.5–5.0 μM concentrations. The limit of detection of ABID for Cu²⁺ could reach at 3.4 × 10^–7 M level, and the quenching constant (K_SV) of ABID towards Cu²⁺ was calculated to be 3.4 × 10⁴ M^–1. The 2:1 stoichiometry and the binding mode between ABID and Cu²⁺ were studied by a Job plot and UV-Vis and fluorescence titration. Additionally, ABID was successfully employed to monitor Cu²⁺ in the Yellow River and tap water samples.

中文翻译：

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1,8-萘二甲酰亚胺-席夫碱衍生的铜（Ii）离子荧光探针：合成，表征和应用

新型荧光探针2-烯丙基-6-（（（2-（（2-羟基-5-硝基苄叉基）氨基）乙基）氨基）-1H-苯并[de]异喹啉-1,3（2H）-二酮（ABID ）是基于萘二甲酰亚胺-席夫碱（Naphthalimide-Schiff base）设计和合成的，用于监测Cu ²⁺离子。在溶液中（DMSO / HEPES，1：1，v / v，pH 7.4），ABID相对于其他重要金属离子显示出对Cu ²⁺离子的荧光猝灭。相关系数（R ²）为0.99的良好线性证明了ABID探针可用于检测0.5–5.0μM浓度的Cu ²⁺离子。Cu ²⁺的ABID的检出限可以达到3.4×10 ^–7 M的水平，并且ABID对Cu的猝灭常数（K _SV）计算得出²⁺为3.4×10 ⁴ M ^–1。通过Job图，UV-Vis和荧光滴定研究了2：1的化学计量和ABID与Cu ²⁺之间的结合模式。此外，ABID已成功用于监测黄河和自来水样品中的Cu ²⁺。