Plants are the major source of a wide array of antimicrobials and antioxidants. Present study describes the biochemical exploration of the methanol and chloroform extracts of aerial parts of Kickxia ramosissima (Wall.) Janchn. Main objective of study was to verify the antimicrobial and antioxidant value of its extracts. The crude extracts were subjected to different biological activities to explore the traditional medicinal potential of this herb. Antibacterial and antifungal activities were determined by using agar well diffusion and tube dilution assays, respectively, while antioxidant activity was investigated by DPPH free radical scavenging assay, phosphomolybdate assay and reducing power assay. The results indicated that both the extracts of aerial parts showed varied degree of antimicrobial, antifungal and antioxidant activities. Overall, the methanol extract showed comparatively better activities in various assays. It revealed significantly higher activity against all the tested bacterial strains (Pseudomonas spp. > Staphylococcus aureus > Staphylococcus epidermidis > Klebsiella pneumoniae > Escherichia coli). A maximum fungicidal activity (54.8%) has shown by methanol extract against Aspergillus terreus. The highest radical scavenging activity by DPPH method was found in case of methanol extracts (49.8% and IC₅₀ 228.53 µg ml⁻¹) at 250 µg ml⁻¹. In phosphomolybdate method, the highest activity was shown by methanolic extract (0.459 nm) at same concentration. Similarly, the highest activity evaluated by reducing power assay was 0.521 nm at the highest concentration of 250 µg ml⁻¹. In conclusion, Kickxia ramosissima is a potential candidate for further studies to explore active compounds particularly responsible for antibacterial and antifungal and activities.

植物是多种抗菌剂和抗氧化剂的主要来源。本研究描述了Kickxia ramosissima地上部分的甲醇和氯仿提取物的​​生化探索。（墙）扬钦。研究的主要目的是验证其提取物的抗菌和抗氧化价值。粗提物经历了不同的生物活性，以探索这种草药的传统药用潜力。分别通过琼脂孔扩散法和试管稀释法测定抗菌和抗真菌活性，而DPPH自由基清除法，磷钼酸盐测定和还原力测定法研究抗氧化活性。结果表明，地上部分提取物均表现出不同程度的抗微生物，抗真菌和抗氧化活性。总体而言，甲醇提取物在各种测定中显示出相对较好的活性。它显示出对所有测试细菌菌株（假单胞菌spp。> 金黄色葡萄球菌 > 表皮葡萄球菌 > 肺炎克雷伯菌 > 大肠杆菌（Escherichia coli））。甲醇提取物对曲霉菌具有最大的杀菌活性（54.8％）。在甲醇提取物（49.8％和IC ₅₀ 228.53 µg ml ^-1）为250 µg ml ^-1的情况下，通过DPPH方法获得的自由基清除活性最高。在磷钼酸法中，相同浓度的甲醇提取物（0.459 nm）显示出最高的活性。同样地，在250μgml ^-1的最高浓度下，通过还原力测定法评价的最高活性为0.521nm 。结论，Kickxia ramosissima是潜在的候选者，可用于进一步研究特别是负责抗菌，抗真菌和活性的活性化合物。