Since Brucella infection mostly occurs through the mucosal surfaces, immune response induced by vaccine that is delivered by a way of mucosal route can be drastically enhanced to control the brucellosis. Omp31is the major outer membrane protein of Brucella, and is considered as a protective antigen against Brucella infection. Accordingly, Lactococcus lactis has been used as an antigen-delivering vector to develop a vaccine-induced mucosal response for having a safer vaccination against brucellosis. A designed omp31 gene fused to the usp45 signal peptide and M6 cell wall anchor was sub cloned in the pNZ7021 expression vector, and a recombinant L. lactis displaying Omp31 was constructed. Omp31 protein expression was confirmed using Western blotting and immunofluorescence analysis. Animals were orally and intraperitoneally immunized with live or killed L. lactis expressing Omp31, respectively. The humoral and cellular immune responses were evaluated by measuring the specific cytokines and antibodies. sIgA, serum IgA, IgM, and total IgG antibodies significantly increased in the mice immunized with live recombinant L. lactis expressing Omp31 and also serum IgM, and total IgG antibodies significantly increased in mice immunized with killed recombinant L. lactis expressing Omp31. Among IgG subtypes, IgG2a response was significantly higher in both groups compared to IgG1. In mice groups immunized with recombinant L. lactis, the IFN-γ and IL-10 level elevated; however, there was no change in the level of IL-4. These results indicated that recombinants L. lactis induce both humoral and cellular immune responses in mice, and also vaccines based on L. lactis-derived live carriers are promising interventions against Brucella melitensis infections.

由于布鲁氏菌感染主要发生在粘膜表面，因此可以通过粘膜途径递送的疫苗诱导的免疫反应可以大大增强，以控制布鲁氏菌病。Omp31是布鲁氏菌的主要外膜蛋白，被认为是针对布鲁氏菌感染的保护性抗原。因此，乳酸乳球菌已被用作抗原递送载体以产生疫苗诱导的粘膜应答，从而具有针对布鲁氏菌病的更安全的疫苗接种。将与usp45信号肽和M6细胞壁锚融合的设计omp31基因亚克隆到pNZ7021表达载体和重组L中。乳酸菌显示Omp31已构建。使用蛋白质印迹和免疫荧光分析确认了Omp31蛋白的表达。用活的或杀死的L.口服和腹膜内免疫动物。球菌分别表达Omp31。通过测量特异性细胞因子和抗体来评估体液和细胞免疫应答。在用活重组L免疫的小鼠中，sIgA，血清IgA，IgM和总IgG抗体显着增加。球菌表达Omp31和也血清IgM和总IgG抗体在用灭活的重组免疫的小鼠显著增加大号。乳酸菌表达Omp31。在IgG亚型中，与IgG1相比，两组的IgG2a反应均显着更高。在小鼠中用重组L免疫。乳酸，IFN-γ和IL-10水平升高；但是，IL-4水平没有变化。这些结果表明，重组大号。乳酸菌在小鼠中诱导体液和细胞免疫反应，还诱导基于L的疫苗。乳酸来源的活载体是对抗布鲁氏菌感染的有前途的干预措施。