Self-supported Fe3N-Co2N nanoarray with high electric conductivity and large surface area was prepared for growth of MIPs and further constructing a sensitive and stable electrochemical sensor. For the evaluation of its performance, Fe3N-Co2N is used as sensing electrode material, and AMP is used as template molecule to construct the MIP electrochemical sensor. Under the optimized conditions, the developed MIPs electrochemical sensor detects AMP with a low detection limit of 3.65 × 10−10 mol L−1 and shows outstanding reproducibility and stability. When the MIPs electrochemical sensor was applied to detect AMP in milk samples via standard addition method, the recovery within 97.06–102.43% with RSD of 1.05–2.11% was obtained. The fabrication of MIPs electrochemical sensor is highly promising for sensitive and selective electrochemical measurement and food safety testing. This work can provide theoretical guidance for truly challenging problems. Graphical abstract Principle diagram of MIP-EC sensor for detecting AMP Molecular imprinted polymers (MIPs) are widely performed for construction of electrochemical (EC) sensors especially for detecting small molecules in complex environment. However, the large-scale and robust preparation of MIPs in situ on sensor platform limits their practical applications. We fabricated a MIPs EC sensor based on Fe3N-Co2N in situ grown on carbon cloth (CC) as the substrate platform (Fe3N-Co2N/CC) combining with MIPs as the target recognition element for the label-free detection of AMP. Under the optimal conditions, the developed MIPs EC sensor can detect AMP with a low detection limit of 3.65 × 10−10 mol L−1. When the AMP in milk is detected by the proposed EC sensor, it shows ideal results. Therefore, the use of self-supported Fe3N-Co2N nanoarray as the platform for the fabrication of MIPs EC sensors is highly promising for sensitive and selective EC measurement and point-of-care testing. Principle diagram of MIP-EC sensor for detecting AMP Molecular imprinted polymers (MIPs) are widely performed for construction of electrochemical (EC) sensors especially for detecting small molecules in complex environment. However, the large-scale and robust preparation of MIPs in situ on sensor platform limits their practical applications. We fabricated a MIPs EC sensor based on Fe3N-Co2N in situ grown on carbon cloth (CC) as the substrate platform (Fe3N-Co2N/CC) combining with MIPs as the target recognition element for the label-free detection of AMP. Under the optimal conditions, the developed MIPs EC sensor can detect AMP with a low detection limit of 3.65 × 10−10 mol L−1. When the AMP in milk is detected by the proposed EC sensor, it shows ideal results. Therefore, the use of self-supported Fe3N-Co2N nanoarray as the platform for the fabrication of MIPs EC sensors is highly promising for sensitive and selective EC measurement and point-of-care testing.

中文翻译：

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使用分子印迹聚合物包覆的 Fe3N-Co2N 纳米阵列对氨苄青霉素进行电化学分析

制备了具有高电导率和大表面积的自支撑 Fe3N-Co2N 纳米阵列，用于 MIP 的生长并进一步构建灵敏稳定的电化学传感器。为评价其性能，以Fe3N-Co2N为传感电极材料，以AMP为模板分子构建MIP电化学传感器。在优化条件下，开发的 MIPs 电化学传感器以 3.65 × 10-10 mol L-1 的低检测限检测 AMP，并显示出出色的重现性和稳定性。当MIPs电化学传感器通过标准添加法检测牛奶样品中的AMP时，回收率为97.06-102.43%，RSD为1.05-2.11%。MIPs 电化学传感器的制造对于灵敏和选择性的电化学测量和食品安全测试非常有前景。这项工作可以为真正具有挑战性的问题提供理论指导。用于检测AMP分子印迹聚合物（MIP）的MIP-EC传感器的图形抽象原理图广泛用于构建电化学（EC）传感器，特别是用于检测复杂环境中的小分子。然而，在传感器平台上原位大规模和稳健地制备 MIP 限制了它们的实际应用。我们制造了一种基于原位生长在碳布（CC）上的 Fe3N-Co2N 作为基底平台（Fe3N-Co2N/CC）结合 MIPs 作为目标识别元件的 MIPs EC 传感器，用于无标记检测 AMP。在最优条件下，开发的 MIPs EC 传感器可以以 3.65 × 10−10 mol L−1 的低检测限检测 AMP。当建议的 EC 传感器检测到牛奶中的 AMP 时，它显示出理想的结果。因此，使用自支撑 Fe3N-Co2N 纳米阵列作为制造 MIPs EC 传感器的平台对于灵敏和选择性的 EC 测量和护理点测试非常有希望。用于检测AMP分子印迹聚合物（MIP）的MIP-EC传感器原理图广泛用于构建电化学（EC）传感器，特别是用于检测复杂环境中的小分子。然而，在传感器平台上原位大规模和稳健地制备 MIP 限制了它们的实际应用。我们制造了一种基于原位生长在碳布（CC）上的 Fe3N-Co2N 作为基底平台（Fe3N-Co2N/CC）结合 MIPs 作为目标识别元件的 MIPs EC 传感器，用于无标记检测 AMP。在最佳条件下，开发的 MIPs EC 传感器可以以 3.65 × 10-10 mol L-1 的低检测限检测 AMP。当建议的 EC 传感器检测到牛奶中的 AMP 时，它显示出理想的结果。因此，使用自支撑 Fe3N-Co2N 纳米阵列作为制造 MIPs EC 传感器的平台对于灵敏和选择性的 EC 测量和护理点测试非常有希望。当建议的 EC 传感器检测到牛奶中的 AMP 时，它显示出理想的结果。因此，使用自支撑 Fe3N-Co2N 纳米阵列作为制造 MIPs EC 传感器的平台对于灵敏和选择性的 EC 测量和护理点测试非常有希望。当建议的 EC 传感器检测到牛奶中的 AMP 时，它显示出理想的结果。因此，使用自支撑 Fe3N-Co2N 纳米阵列作为制造 MIPs EC 传感器的平台对于灵敏和选择性的 EC 测量和护理点测试非常有希望。