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Electrochemical assay of ampicillin using Fe3N-Co2N nanoarray coated with molecularly imprinted polymer

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Abstract

Self-supported Fe3N-Co2N nanoarray with high electric conductivity and large surface area was prepared for growth of MIPs and further constructing a sensitive and stable electrochemical sensor. For the evaluation of its performance, Fe3N-Co2N is used as sensing electrode material, and AMP is used as template molecule to construct the MIP electrochemical sensor. Under the optimized conditions, the developed MIPs electrochemical sensor detects AMP with a low detection limit of 3.65 × 10−10 mol L−1 and shows outstanding reproducibility and stability. When the MIPs electrochemical sensor was applied to detect AMP in milk samples via standard addition method, the recovery within 97.06–102.43% with RSD of 1.05–2.11% was obtained. The fabrication of MIPs electrochemical sensor is highly promising for sensitive and selective electrochemical measurement and food safety testing. This work can provide theoretical guidance for truly challenging problems.

Principle diagram of MIP-EC sensor for detecting AMP

Molecular imprinted polymers (MIPs) are widely performed for construction of electrochemical (EC) sensors especially for detecting small molecules in complex environment. However, the large-scale and robust preparation of MIPs in situ on sensor platform limits their practical applications. We fabricated a MIPs EC sensor based on Fe3N-Co2N in situ grown on carbon cloth (CC) as the substrate platform (Fe3N-Co2N/CC) combining with MIPs as the target recognition element for the label-free detection of AMP. Under the optimal conditions, the developed MIPs EC sensor can detect AMP with a low detection limit of 3.65 × 10−10 mol L−1. When the AMP in milk is detected by the proposed EC sensor, it shows ideal results. Therefore, the use of self-supported Fe3N-Co2N nanoarray as the platform for the fabrication of MIPs EC sensors is highly promising for sensitive and selective EC measurement and point-of-care testing.

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Funding

This study is supported by the National Natural Science Foundation of China 21775053, the Natural Science Foundation of Shandong Province (No. 2019GSF111023, ZR2017MB027), and the National Key Scientific Instrument and Equipment Development Project of China (No. 21627809). Yong Zhang is sponsored by the China Scholarship Council (CSC), and Y. G. W. thanks the Program for Scientific Research Innovation Team in Colleges and Universities of Shandong Province.

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Authors and Affiliations

  1. Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People’s Republic of China

    Zhaoyi Liu, Yong Zhang, Xiang Ren & Qin Wei

  2. Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People’s Republic of China

    Zhaoyi Liu, Yong Zhang, Hongmin Ma & Qin Wei

  3. Culinary Institute, University of Jinan, Jinan, 250022, People’s Republic of China

    Tao Fan

  4. Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, People’s Republic of China

    Yaoguang Wang

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  1. Zhaoyi Liu
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Correspondence to Yong Zhang.

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Liu, Z., Fan, T., Zhang, Y. et al. Electrochemical assay of ampicillin using Fe3N-Co2N nanoarray coated with molecularly imprinted polymer. Microchim Acta 187, 442 (2020). https://doi.org/10.1007/s00604-020-04432-2

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