Recent observations of increasing incidences of potato plants showing blackleg symptoms have caused serious concern for potato growers and traders in Egypt. Potato plants showing blackleg and soft rot symptoms were sampled from main potato-growing areas in Egypt during three successive seasons. Dickeya spp., Pectobacterium carotovorum and Pectobacterium atrosepticum were successfully isolated and identified from symptomatic potato plants (tubers and stems). The identity of bacterial isolates was reviled by identification methods based on different biological principles: cultural, biochemical and molecular methods. Specific primers targeting different genomic loci were selected for molecular identification, utilizing either conventional or real-time PCR. Multiple species were isolated from some infected samples revealing complex infection, which confirm the complexity and interaction of soft rot and blackleg diseases. Given that no previous reports on Dickeya diseases in Egypt were confirmed since the recent revision of its taxonomy, the result of PCR with Dickeya spp. was furtherly confirmed using DNA sequencing assay. BLASTn analyses of 16S rDNA sequences showed similarity to several species of Dickeya, with 99% nucleotide similarity to strains of, D. chrysanthemi, D. dianthicola and D. solani, respectively. Furthermore, the most commonly identified bacterial pathogen in the tested samples was P. carotovorum followed by P. atrosepticum and Dickeya spp., while P. brasiliense was never detected in any of the collected samples confirmed by real-time PCR assay. The results of such field survey suggested to help the decision makers in Egypt to establish an action plan to face the increasing risk of potato diseases caused by Dickeya spp. for the benefit of Egyptian domestic production.

最近出现黑腿症状的马铃薯植物发病率上升的观察结果引起了埃及马铃薯种植者和商人的严重关注。在连续三个季节中，从埃及主要的马铃薯种植区采样了显示出黑腿病和软腐病症状的马铃薯植株。Dickeya spp 。，Carotovorum菌和atrosepticum菌从有症状的马铃薯植物（块茎和茎）中成功分离和鉴定。通过基于不同生物学原理的鉴定方法（培养，生化和分子方法），细菌分离株的身份受到了谴责。利用常规或实时PCR选择靶向不同基因组位点的特异性引物进行分子鉴定。从一些感染样本中分离出多种物种，显示出复杂的感染，这证实了软腐病和黑腿病的复杂性和相互作用。鉴于自从最近对其分类法进行修订以来，尚未确认埃及有关迪基亚病的任何报道，因此用迪基亚PCR的结果spp。使用DNA测序测定进一步证实。对16S rDNA序列进行BLASTn分析表明，它与Dickeya的几种物种具有相似性，分别与D. chrysanthemi，D。dianthicola和D. solani菌株具有99％的核苷酸相似性。此外，在测试样品中最常鉴定出的细菌病原体是胡萝卜假单胞菌，其次是atrosepticum和Dickeya spp。，而巴西假单胞菌实时荧光定量PCR确证的任何采集样品中均未检出任何SNP。此类实地调查的结果建议帮助埃及的决策者制定一项行动计划，以应对由迪卡氏菌引起的马铃薯疾病增加的风险。为了埃及国内生产的利益。