This study investigated and compared the microbial communities between a sequencing batch reactor (SBR) without carriers and a hybrid SBR with addition of carriers for the treatment of saline wastewater. The two systems were operated over 292 days with alternating aerobic/anoxic mode (temperature: 28℃, salinity: 0.0–3.0%). High removal efficiency of chemical oxygen demand (COD) and total inorganic nitrogen (TIN) was achieved in both the SBR (above 86.7 and 95.4% respectively) and hybrid SBR (above 84.4 and 94.0%) at 0.0–2.5% salinity. Further increasing salinity to 3.0% decreased TIN removal efficiency to 78.4% in the hybrid SBR. Steep decline of biodiversity and relative abundance of ammonia-oxidizing bacteria (AOB) contributed to the worse performance. More genera related to sulfide-oxidizing and sulfate-reducing bacteria were detected in the hybrid SBR than the SBR at 3.0% salinity. The abundance of halotolerant bacteria increased with the salinity increase for both reactors, summing up to 25.5% in the suspended sludge (S-sludge) from the SBR, 28.9 and 22.9% in the S-sludge and biofilm taken from the hybrid SBR, respectively. Nitrification and denitrification via nitrate was the main nitrogen removal pathway in the SBR and hybrid SBR at 0.0 and 0.5% salinity, while partial nitrification and denitrification via nitrite became the key process for nitrogen removal in the two reactors when the salinity was increased to 1.0–3.0%. Higher abundance of anaerobic ammonium-oxidizing (ANAMMOX) and sulfide-oxidizing autotrophic denitrification (SOAD) bacteria were found in the hybrid SBR at 3.0% salinity.

本研究调查并比较了无载体序批式反应器 (SBR) 和添加载体的混合 SBR 之间的微生物群落，用于处理含盐废水。两个系统以交替的有氧/缺氧模式运行超过 292 天（温度：28℃，盐度：0.0-3.0%）。在 0.0-2.5% 的盐度下，SBR（分别高于 86.7% 和 95.4%）和混合 SBR（高于 84.4% 和 94.0%）都实现了化学需氧量 (COD) 和总无机氮 (TIN) 的高去除效率。在混合 SBR 中，进一步将盐度增加到 3.0%，将 TIN 去除效率降低到 78.4%。生物多样性的急剧下降和氨氧化细菌 (AOB) 的相对丰度导致性能下降。在盐度为 3.0% 的情况下，杂交 SBR 中检测到更多与硫化物氧化和硫酸盐还原细菌相关的属。耐盐细菌的丰度随着两个反应器盐度的增加而增加，在 SBR 的悬浮污泥（S-污泥）中总计高达 25.5%，在来自混合 SBR 的 S-污泥和生物膜中分别高达 28.9% 和 22.9% . 0.0%和0.5%盐度下SBR和杂化SBR的主要脱氮途径是硝酸盐硝化反硝化，而当盐度提高到1.0-时，亚硝酸盐部分硝化反硝化成为两个反应器脱氮的关键过程。 3.0%。在 3.0% 盐度的混合 SBR 中发现了更高丰度的厌氧氨氧化 (ANAMMOX) 和硫化物氧化自养反硝化 (SOAD) 细菌。