A marine-sediment-derived bacterium Bacillus velezensis SH-B74 can produce cyclic lipopeptides (CLPs). This study presented the isolation, characterization, and activity evaluation of a new CLP from the bacterial cultures of the strain SH-B74. Multiple chromatographic methods (solid-phase extraction and reversed-phase high-performance liquid chromatography) were applied to the purifying procedure of CLP, and the structural characterization of the new CLP was conducted by various spectroscopy (1D and 2D nuclear magnetic resonance together with Fourier transform infrared spectroscopy) and spectrometry (liquid chromatography-mass spectrometry, high-resolution mass spectrometry and tandem mass spectrometry) techniques as well as Marfey’s method. The results displayed that the new CLP (anteiso-C₁₅ Ile_2,7 surfactin, 1) consists of a peptidic backbone of l-Glu₁, l-Ile₂, d-Leu₃, l-Val₄, l-Asp₅, d-Leu₆, l-Ile₇, and an anteiso-C₁₅ type saturated fatty acid chain. Further activity assay showed that the new CLP displays activity on the inhibition of the appressoria formation of rice blast causal pathogen Magnaporthe oryzae. To sum up, the results presented the perspective of potential application of the new CLP as a green agrichemical to control M. oryzae.

海洋沉淀来源的细菌velezensis SH-B74可以产生环状脂肽（CLP）。这项研究提出了从菌株SH-B74的细菌培养物中分离出的新型CLP的分离，表征和活性评估。将多种色谱方法（固相萃取和反相高效液相色谱）应用于CLP的纯化过程，并通过各种光谱（一维和二维核磁共振以及傅立叶核磁共振）对新的CLP进行结构表征变换红外光谱）和光谱分析（液相色谱-质谱，高分辨率质谱和串联质谱）技术以及Marfey方法。结果显示，新的CLP（反异构-C ₁₅ Ile _2,7 surfactin，1）由l -Glu ₁，l -Ile ₂，d -Leu ₃，l -Val ₄，l -Asp ₅，d -Leu ₆，l -Ile的肽骨架组成₇，和反异-C ₁₅类型的饱和脂肪酸链。进一步的活性​​测定表明，新的CLP对抑制稻瘟病病原体稻瘟病菌的感官形成具有抑制作用。。综上所述，结果提供了将新型CLP作为绿色农业化学品控制M的潜在应用前景。菌。