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Isolation and characterization of a new cyclic lipopeptide surfactin from a marine-derived Bacillus velezensis SH-B74

The Journal of Antibiotics volume 73, pages 863–867 (2020)Cite this article

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Abstract

A marine-sediment-derived bacterium Bacillus velezensis SH-B74 can produce cyclic lipopeptides (CLPs). This study presented the isolation, characterization, and activity evaluation of a new CLP from the bacterial cultures of the strain SH-B74. Multiple chromatographic methods (solid-phase extraction and reversed-phase high-performance liquid chromatography) were applied to the purifying procedure of CLP, and the structural characterization of the new CLP was conducted by various spectroscopy (1D and 2D nuclear magnetic resonance together with Fourier transform infrared spectroscopy) and spectrometry (liquid chromatography-mass spectrometry, high-resolution mass spectrometry and tandem mass spectrometry) techniques as well as Marfey’s method. The results displayed that the new CLP (anteiso-C15 Ile2,7 surfactin, 1) consists of a peptidic backbone of l-Glu1, l-Ile2, d-Leu3, l-Val4, l-Asp5, d-Leu6, l-Ile7, and an anteiso-C15 type saturated fatty acid chain. Further activity assay showed that the new CLP displays activity on the inhibition of the appressoria formation of rice blast causal pathogen Magnaporthe oryzae. To sum up, the results presented the perspective of potential application of the new CLP as a green agrichemical to control M. oryzae.

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References

  1. Ongena M, Jacques P. Bacillus lipopeptides: versatile weapons for plant disease biocontrol. Trends Microbiol. 2008;16:115–25.

    Article  CAS  PubMed  Google Scholar 

  2. Cochrane SA, Vederas JC. Lipopeptides from Bacillus and Paenibacillus spp.: a gold mine of antibiotic candidates. Med Res Rev. 2016;36:4–31.

    Article  CAS  PubMed  Google Scholar 

  3. Farace G, Fernandez O, Jacquens L, Coutte F, Krier F, Jacques P, Clément C, Barka EA, Jacquard C, Dorey S. Cyclic lipopeptides from Bacillus subtilis activate distinct patterns of defence responses in grapevine. Mol Plant Pathol. 2015;16:177–87.

    Article  CAS  PubMed  Google Scholar 

  4. Niu L, Nan W, Wang X, Hu J, Wang S. Screening and identification of a deep-sea derived Bacillus sp. producing lipopeptide bacillopeptin A. Chin J Antibiot. 2011;10:738–44.

    Google Scholar 

  5. Ma Z, Hu J. Plipastatin A1 produced by a marine sediment-derived Bacillus amyloliquefaciens SH-B74 contributes to the control of gray mold disease in tomato. 3 Biotech. 2018;8:125.

    Article  PubMed  PubMed Central  Google Scholar 

  6. Ma Z, Hu J, Wang X, Wang S. NMR spectroscopic and MS/MS spectrometric characterization of a new lipopeptide antibiotic bacillopeptin B1 produced by a marine sediment-derived Bacillus amyloliquefaciens SH-B74. J Antibiot. 2013;67:175–8.

    Article  Google Scholar 

  7. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. New York, NY: Cold Spring Harbor Laboratory Press; 1989.

    Google Scholar 

  8. Ma Z, Hu J. Production and characterization of surfactin-type lipopeptides as bioemulsifiers produced by a Pinctada martensii-derived Bacillus mojavensis B0621A. Appl Biochem Biotechnol. 2015;177:1520–9.

    Article  CAS  PubMed  Google Scholar 

  9. Tang J, Zhao F, Gao H, Dai Y, Yao Z, Hong K, Li J, Ye W, Yao X. Characterization and online detection of surfactin isomers based on HPLC-MSn analyses and their inhibitory effects on the overproduction of nitric oxide and the release of TNF-α and IL-6 in LPS-induced macrophages. Mar Drugs. 2010;8:2605–18.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Pagadoy M, Peypoux F, Wallach J. Solid-phase synthesis of surfactin, a powerful biosurfactant produced by Bacillus subtilis, and of four analogues. Int J Pept Res Ther. 2005;11:195–202.

    Article  CAS  Google Scholar 

  11. Pang X, Zhao J, Fang X, Liu H, Zhang Y, Cen S, Yu L. Surfactin derivatives from Micromonospora sp. CPCC 202787 and their anti-HIV activities. J Antibiot. 2016;70:105–8.

    Article  Google Scholar 

  12. Ma Z, Wang N, Hu J, Wang S. Isolation and characterization of a new iturinic lipopeptide, mojavensin A produced by a marine-derived bacterium Bacillus mojavensis B0621A. J Antibiot. 2012;65:317–22.

    Article  CAS  Google Scholar 

  13. Ma Z, Geudens N, Kieu NP, Sinnaeve D, Ongena M, Martins JC, Höfte M. Biosynthesis, chemical structure, and structure-activity relationship of orfamide lipopeptides produced by Pseudomonas protegens and related species. Front Microbiol. 2016;7:382.

    PubMed  PubMed Central  Google Scholar 

  14. Rebollar A, Lopez-Garcia B. PAF104, a synthetic peptide to control rice blast disease by blocking appressorium formation in Magnaporthe oryzae. Mol Plant-Microbe Interact. 2013;26:1407–16.

    Article  CAS  PubMed  Google Scholar 

  15. Wu L, Xiao W, Chen G, Song D, Khaskheli MA, Li P, Zhang S, Feng G. Identification of Pseudomonas mosselii BS011 gene clusters required for suppression of Rice Blast Fungus Magnaporthe oryzae. J Biotechnol. 2018;20:1–9.

    Article  Google Scholar 

Download references

Acknowledgements

Research grants from the Project for Enhancing the Research Capability of Young Teachers in Northwest Normal University (given to Z.M., no. NWNU-LKQN-18-26) and from the National Science Foundation of China (given to J.H., no. 41776178) are acknowledged for supporting this study.

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Authors and Affiliations

  1. College of Life Science, Northwest Normal University, 967 East Anning Road, 730070, Lanzhou, China

    Zongwang Ma, Shihu Zhang, Guoyang Wu, Yue Shao, Quanfeng Mi, Junyu Liang & Kun Sun

  2. Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, 1068 Xueyuan Road, 518055, Shenzhen, China

    Songya Zhang

  3. Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, 110016, Shenyang, China

    Jiangchun Hu

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  1. Zongwang Ma
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Contributions

Z.M. conceived, designed, and conducted all the experiments. Z.M. wrote the manuscript. Z.M., Songya Z., Shihu Z., J.L., K.S., and J.H. together improved the manuscript and approved the final version of the manuscript for submission. G.W., Y.S., and Q.M. were involved in the revision process of the manuscript.

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Correspondence to Zongwang Ma or Jiangchun Hu.

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Ma, Z., Zhang, S., Zhang, S. et al. Isolation and characterization of a new cyclic lipopeptide surfactin from a marine-derived Bacillus velezensis SH-B74. J Antibiot 73, 863–867 (2020). https://doi.org/10.1038/s41429-020-0347-9

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