microRNAs are small non-coding RNA molecule that plays an important role in metabolism. Chrysopogon zizanioides (L.) Roberty is an important aromatic plant used in perfumery industries, soil, water conservation, and agricultural practices. In this study, the transcriptomic sequence of vetiver leaf and root was subjected to miRNA identification by the computational methods. miRNA identification was carried out using a homology-based method by C-mii software with several other online tools. A total of 80 miRNA were identified from both leaf and root sequences. Target identification was done by identified miRNA sets. A total of 25 and 31 miRNA families were identified in both leaf and root, respectively, with ten common families involve in different ontological function. miR169 and miR5021 regulate most of the target in leaf and root. In vetiver, many primary and secondary metabolism elements are regulated by miRNA as photo-system, transcription factor, terpenoid metabolism, etc. Here is the first in silico study revealing the specific miRNAs and their target genes for corresponding root and leaf tissues respectively in C. zizanioides.

microRNA是小的非编码RNA分子，在代谢中起重要作用。金枪鱼（L.）Roberty是重要的芳香植物，用于香料工业，土壤，节水和农业实践。在这项研究中，香根草叶和根的转录组序列通过计算方法进行了miRNA鉴定。通过基于同源性的方法，通过C-mii软件和其他几种在线工具进行miRNA鉴定。从叶和根序列中总共鉴定出80个miRNA。通过鉴定的miRNA集进行靶标鉴定。在叶和根中分别鉴定出总共25和31个miRNA家族，其中10个共同的家族涉及不同的本体功能。miR169和miR5021调节叶和根中的大多数靶标。在香根草中，许多主要和次要代谢元素都受miRNA调节，例如光系统，转录因子，C. zizanioides。