Peroxisome proliferator-activated receptors (PPARs) play roles in glucose and lipid metabolism regulation. Pro12Ala PPAR-γ2 and +294T/C PPAR-δ have been associated with dyslipidemia, hyperglycemia and high body mass index (BMI). We compared metabolic traits and determined associations with Pro12Ala PPAR-γ2 or +294T/C PPAR-δ polymorphism among teenagers from different ethnicity. Four hundred and twelve samples with previous biochemical and biometric measurements were used. Genomic DNA from peripheral blood was extracted and analyzed by end-point PCR for Pro12Ala PPAR-γ2. The +294T/C PPAR-δ PCR product was also digested with Bsl I. Two genotype groups were formed: major allele homozygous and minor allele carriers. Pro12Ala PPAR-γ2 G minor allele frequencies were: 10% in Mestizo-1, 19% in Mestizo-2, 23% in Tarahumara, 12% in Mennonite, and 17% in the total studied population. The +294T/C PPAR-δ C minor allele frequencies were: 18% in Mestizo-1, 20% in Mestizo-2, 6% in Tarahumara, 13% in Mennonite, and 12% in the total studied population. Teenagers with PPAR-γ2 G allele showed a greater risk for either high waist/height ratio or low high-density lipoprotein; and, also had lower total cholesterol. Whereas, PPAR-γ2 G allele showed lower overweight/obesity phenotype (BMI Z-score) frequency, PPAR-δ C allele was a risk factor for it. Metabolic traits were associated with both PPAR polymorphisms.

中文翻译：

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Pro12Ala PPAR-γ2 和 +294T/C PPAR-δ 多态性及其与墨西哥北部青少年代谢特征的关联

过氧化物酶体增殖物激活受体 (PPAR) 在葡萄糖和脂质代谢调节中发挥作用。Pro12Ala PPAR-γ2 和 +294T/C PPAR-δ 与血脂异常、高血糖和高体重指数 (BMI) 相关。我们比较了不同种族青少年的代谢特征并确定了与 Pro12Ala PPAR-γ2 或 +294T/C PPAR-δ 多态性的关联。使用了具有先前生化和生物特征测量的四百一十二个样本。从外周血中提取基因组 DNA 并通过终点 PCR 分析 Pro12Ala PPAR-γ2。+294T/C PPAR-δ PCR产物也用Bsl I消化。形成两个基因型组：主要等位基因纯合子和次要等位基因携带者。Pro12Ala PPAR-γ2 G 次要等位基因频率为：Mestizo-1 为 10%，Mestizo-2 为 19%，Tarahumara 为 23%，Mennonite 为 12%，占总研究人群的 17%。+294T/C PPAR-δ C 次要等位基因频率为：在 Mestizo-1 中为 18%，在 Mestizo-2 中为 20%，在 Tarahumara 中为 6%，在 Mennonite 中为 13%，在总研究人群中为 12%。具有 PPAR-γ2 G 等位基因的青少年出现高腰围/身高比或低高密度脂蛋白的风险更大；而且，总胆固醇也较低。然而，PPAR-γ2 G 等位基因显示出较低的超重/肥胖表型（BMI Z 分数）频率，而 PPAR-δ C 等位基因是其危险因素。代谢性状与两种 PPAR 多态性相关。具有 PPAR-γ2 G 等位基因的青少年出现高腰围/身高比或低高密度脂蛋白的风险更大；而且，总胆固醇也较低。然而，PPAR-γ2 G 等位基因显示出较低的超重/肥胖表型（BMI Z 分数）频率，而 PPAR-δ C 等位基因是其危险因素。代谢性状与两种 PPAR 多态性相关。具有 PPAR-γ2 G 等位基因的青少年出现高腰围/身高比或低高密度脂蛋白的风险更大；而且，总胆固醇也较低。然而，PPAR-γ2 G 等位基因显示出较低的超重/肥胖表型（BMI Z 分数）频率，而 PPAR-δ C 等位基因是其危险因素。代谢性状与两种 PPAR 多态性相关。