OBJECTIVE To investigate a possible correlation between chromosomal aberrations and paternal age, analyzing embryos derived from young oocyte donors, with available preimplantation genetic testing for aneuploidy results from day 5/6 trophectoderm biopsy obtained by next-generation sequencing for all 24 chromosomes. DESIGN Retrospective cohort study. SETTING Canadian fertility centre. PATIENT(S) A total of 3,118 embryos from 407 male patients, allocated into three paternal age groups: group A, ≤39 years (n = 203); group B, 40-49 years (n = 161); group C, ≥50 years (n = 43). INTERVENTION(S) None. MAIN OUTCOME MEASURE(S) The primary outcomes were aneuploidy, euploidy, mosaicism, and blastocyst formation rates. Secondary endpoints were comparison of specific chromosome aneuploidy, segmental and complex (involving two chromosomes + mosaicism >50%) aneuploidy, and analysis of overall percentage of chromosomal gains and losses within each group. RESULT(S) The study included 437 in vitro fertilization (IVF) antagonist cycles using 302 oocyte donors in which preimplantation genetic testing for aneuploidy was performed. Overall, 70.04% of embryos were euploid, 13.9% were aneuploid, and 16.06% were mosaic. No significant differences among paternal age groups A, B, and C were found in euploidy rates (69.2%, 70.6%, 71.4%, respectively), aneuploidy rates (14.7%, 12.8%, 13.9%, respectively) or mosaicism rates (16.1%, 16.6%, 13.6%; respectively). The fertilization rate was lower in group C compared with group B (76.35% vs. 80.09%). No difference was found in blastocyst formation rate between the study groups (median 52% [interquartile range, 41%, 67%] vs. 53% [42%, 65%] vs. 52% [42%, 64%], respectively). A generalized linear mixed model regression analysis for embryo ploidy rates found older oocyte donor age to be independently associated with embryo aneuploidy (odds ratio = 1.041; 95% CI, 1.009-1.074). The rate of segmental aneuploidies was significantly higher in the older versus younger paternal age group (36.6% vs. 19.4%). CONCLUSION(S) No association was found between paternal age and aneuploidy rates in embryos derived from IVF cycles using young oocyte donors, after adjusting for donor, sperm, and IVF cycle characteristics. Advanced paternal age ≥ 50, compared with younger paternal ages, was associated with a lower fertilization rate and increased rate of segmental aberrations.

中文翻译：

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父亲年龄和非整倍体率之间是否存在相关性？对来自年轻卵子捐赠者的 3,118 个胚胎的分析

目的 研究染色体畸变与父亲年龄之间可能存在的相关性，分析来自年轻卵母细胞供体的胚胎，并利用对所有 24 条染色体进行下一代测序获得的第 5/6 天滋养外胚层活检的非整倍体结果进行植入前基因检测。设计 回顾性队列研究。设置加拿大生育中心。PATIENT(S) 来自 407 名男性患者的总共 3,118 个胚胎，分为三个父亲年龄组：A 组，≤39 岁（n = 203）；B 组，40-49 岁（n = 161）；C 组，≥50 岁（n = 43）。干预措施 无。主要结局指标 主要结局是非整倍体、整倍体、嵌合体和囊胚形成率。次要终点是特定染色体非整倍性、节段性和复杂性（涉及两条染色体 + 嵌合 > 50%) 非整倍性，并分析每组内染色体获得和损失的总体百分比。结果 该研究包括 437 个体外受精 (IVF) 拮抗剂周期，使用 302 个卵母细胞供体进行了非整倍体的植入前基因检测。总体而言，70.04% 的胚胎为整倍体，13.9% 为非整倍体，16.06% 为嵌合体。父年龄组 A、B 和 C 在整倍体率（分别为 69.2%、70.6%、71.4%）、非整倍体率（分别为 14.7%、12.8%、13.9%）或嵌合率（16.1）方面没有发现显着差异%、16.6%、13.6%；分别）。C组受精率低于B组（76.35% vs. 80.09%）。研究组之间的囊胚形成率没有差异（中位数 52% [四分位距，41%, 67%] vs. 53% [42%, 65%] vs. 52% [42%, 64%]，分别）。胚胎倍性率的广义线性混合模型回归分析发现，年龄较大的卵母细胞供体年龄与胚胎非整倍性独立相关（优势比 = 1.041；95% CI，1.009-1.074）。与年轻父亲年龄组相比，年长组的节段性非整倍体发生率显着更高（36.6% 对 19.4%）。结论（S）在调整供体、精子和 IVF 周期特征后，未发现父亲年龄与使用年轻卵母细胞供体的 IVF 周期衍生的胚胎的非整倍体率之间存在关联。与年轻的父亲年龄相比，年龄大于 50 岁的父亲与较低的受精率和节段性畸变率增加有关。胚胎倍性率的广义线性混合模型回归分析发现，年龄较大的卵母细胞供体年龄与胚胎非整倍性独立相关（优势比 = 1.041；95% CI，1.009-1.074）。与年轻父亲年龄组相比，年长组的节段性非整倍体发生率显着更高（36.6% 对 19.4%）。结论（S）在调整供体、精子和 IVF 周期特征后，未发现父亲年龄与使用年轻卵母细胞供体的 IVF 周期衍生的胚胎的非整倍体率之间存在关联。与年轻的父亲年龄相比，年龄大于 50 岁的父亲与较低的受精率和节段性畸变率增加有关。胚胎倍性率的广义线性混合模型回归分析发现，年龄较大的卵母细胞供体年龄与胚胎非整倍性独立相关（优势比 = 1.041；95% CI，1.009-1.074）。与年轻父亲年龄组相比，年长组的节段性非整倍体发生率显着更高（36.6% 对 19.4%）。结论（S）在调整供体、精子和 IVF 周期特征后，未发现父亲年龄与使用年轻卵母细胞供体的 IVF 周期衍生的胚胎的非整倍体率之间存在关联。与年轻的父亲年龄相比，年龄大于 50 岁的父亲与较低的受精率和节段性畸变率增加有关。与年轻父亲年龄组相比，年长组的节段性非整倍体发生率显着更高（36.6% 对 19.4%）。结论（S）在调整供体、精子和 IVF 周期特征后，未发现父亲年龄与使用年轻卵母细胞供体的 IVF 周期衍生的胚胎的非整倍体率之间存在关联。与年轻的父亲年龄相比，年龄大于 50 岁的父亲与较低的受精率和节段性畸变率增加有关。与年轻父亲年龄组相比，年长组的节段性非整倍体发生率显着更高（36.6% 对 19.4%）。结论（S）在调整供体、精子和 IVF 周期特征后，未发现父亲年龄与使用年轻卵母细胞供体的 IVF 周期衍生的胚胎的非整倍体率之间存在关联。与年轻的父亲年龄相比，年龄大于 50 岁的父亲与较低的受精率和节段性畸变率增加有关。