This study aimed to investigate the effect of black cumin seed oil (BCSO) on the growth metrics, innate immunity and resistance against Pseudomonas fluorescens infection in Nile tilapia Oreochromis niloticus. This was done by conducting a feeding trial experiment on the fish. A total of four diets (protein, 35.99 ± 0.16%; lipid, 9.86 ± 0.06%) varying in their BCSO ration (w/w; 0% (control), 1%, 2% or 4%) were formulated. A total of 180 fish (25.6 ± 2.2 g fish^─1) were allocated to four experimental groups with three replicates (twelve 150-L glass-tanks; fifteen fish tank^─1). Each fish group was fed thrice a day (8:00, 12:00 and 16:00 h) with one of the four diets at 3.5% of their body weight for 28 days. Following day 7, 14 and 28 of the feeding trial, the growth metrics (i.e. weight gain (WG), specific growth rate (SGR) and food conversion ratio (FCR)) were measured based on bulk weight of the fish tank^─1, and the immune parameters (i.e. serum-bactericidal activity (SBA), respiratory burst activity (RBA) and phagocytic activity (PA)) were examined by analysing blood collected from a randomly selected representative fish tank^─1. Following the feeding trial course, the fish were injected with P. fluorescens at day 29 and the cumulative mortality was recorded until day 30 post-injection. Obtained results did not show any suppressive effect of BCSO on growth, and showed positive effects on examined immune parameters and survival of all the BCSO-fed fish groups, with best results in 2% BCSO-fed group. At the end of the feeding trial (day 28), SBA, PA and RBA in 2% BCSO-fed fish were increased by 3.66-, 1.94- and 2.85-fold, respectively, when compared to the control. In the same group of fish, the maximum significant (P < 0.05) reduction of post-challenge cumulative mortality (43.33%) was recorded when the mortality was 100% in the control group. Accordingly, this study suggests the dietary inclusion of BCSO at 2% towards controlling P. fluorescens infection in O. niloticus.

本研究的目的是调查黑孜然籽油（BCSO）对生长的度量，先天免疫和抗效果荧光假单胞菌感染尼罗罗非鱼尼罗罗非鱼。这是通过对鱼进行饲喂试验来完成的。配制了四种BCSO比例（w / w； 0％（对照），1％，2％或4％）变化的饮食（蛋白质，35.99±0.16％；脂质，9.86±0.06％）。总共将180条鱼（25.6±2.2 g鱼^─1）分配给四个实验组，每组三个重复（十二个150 L^玻璃罐；十五个鱼缸^─1））。每个鱼群一天（8：00、12：00和16:00 h）三次喂食四种饮食之一，体重占其体重的3.5％，持续28天。在喂养试验的第7、14和28天之后，根据鱼缸的^总重量^─1来衡量生长指标（即体重增加（WG），比生长率（SGR）和食物转化率（FCR）），通过分析从随机选择的代表性鱼缸^─1中采集的血液来检查免疫参数（即血清杀菌活性（SBA），呼吸爆发活性（RBA）和吞噬活性（PA））。在饲养试验过程之后，给鱼注射了荧光假单胞菌在注射后第29天记录累积死亡率，直到注射后第30天。获得的结果没有显示BCSO对生长的任何抑制作用，并且显示了对所有BCSO喂养的鱼组的检查的免疫参数和存活的积极影响，其中以2％BCSO喂养的组的最佳结果。在饲养试验结束时（第28天），与对照组相比，在2％BCSO喂养的鱼中SBA，PA和RBA分别增加了3.66倍，1.94倍和2.85倍。在同一组鱼中， 当对照组的死亡率为100％时，记录到其攻击后累积死亡率的最大显着降低（P <0.05）（43.33％）。因此，这项研究表明，在饮食中添加2％的BCSO有助于控制尼罗罗非鱼的荧光假单胞菌感染。。