Gold nanoparticles (AuNPs) conjugated with Cy3-tagged aptamer which can specifically recognize chloramphenicol (CAP) (referred to as AuNPs-AptCAP) are described. CAP can trigger the configuration change of CAP binding aptamer, and thus switching the fluorescence of AuNPs-AptCAP through changing the efficiency of the fluorescence resonance energy transfer (FRET) system with Cy3 as donors and AuNPs as recipients. AuNPs-AptCAP exhibits a linear range of CAP concentrations from 26.0 to 277 μg L −1 with a limit of detection of 8.1 μg L −1 when Cy3 was excited at 530 nm and emission was measured at 570 nm. More importantly, AuNPs-AptCAP can be utilized as signal transducers for the build-up of a series of logic gates including YES, PASS 0, INH, NOT, PASS 1, and NAND. Utilizing the principle of a metal ion–mediated fluorescence switch together with a strong metal ion chelator, the fluorescence of AuNPs-AptCAP could be modulated by adding metal ions and EDTA sequentially. Therefore, a “Plug and Play” logic system based on AuNPs-AptCAP has been realized by simply adding other components to create new logic functions. This work highlights the advantages of simple synthesis and facile fluorescence switching properties, which will provide useful knowledge for the establishment of molecular logic systems. Graphical abstract

中文翻译：

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“即插即用”逻辑门构建基于与 Cy3 标记的适体结合的金纳米粒子的化学触发荧光切换

描述了与可以特异性识别氯霉素 (CAP) 的 Cy3 标记的适体结合的金纳米粒子 (AuNPs)（称为 AuNPs-AptCAP）。CAP可以触发CAP结合适体的构型变化，从而通过改变以Cy3作为供体和AuNPs作为受体的荧光共振能量转移（FRET）系统的效率来切换AuNPs-AptCAP的荧光。当 Cy3 在 530 nm 处激发并在 570 nm 处测量发射时，AuNPs-AptCAP 表现出 26.0 至 277 μg L -1 的线性范围 CAP 浓度，检测限为 8.1 μg L -1 。更重要的是，AuNPs-AptCAP 可用作信号传感器，用于构建一系列逻辑门，包括 YES、PASS 0、INH、NOT、PASS 1 和 NAND。利用金属离子介导的荧光开关和强金属离子螯合剂的原理，可以通过依次添加金属离子和 EDTA 来调节 AuNPs-AptCAP 的荧光。因此，通过简单地添加其他组件来创建新的逻辑功能，实现了基于 AuNPs-AptCAP 的“即插即用”逻辑系统。这项工作突出了简单合成和容易的荧光转换特性的优点，这将为分子逻辑系统的建立提供有用的知识。图形概要 这项工作突出了简单合成和容易的荧光转换特性的优点，这将为分子逻辑系统的建立提供有用的知识。图形概要 这项工作突出了简单合成和容易的荧光转换特性的优点，这将为分子逻辑系统的建立提供有用的知识。图形概要