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“Plug and Play” logic gate construction based on chemically triggered fluorescence switching of gold nanoparticles conjugated with Cy3-tagged aptamer

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Abstract

Gold nanoparticles (AuNPs) conjugated with Cy3-tagged aptamer which can specifically recognize chloramphenicol (CAP) (referred to as AuNPs-AptCAP) are described. CAP can trigger the configuration change of CAP binding aptamer, and thus switching the fluorescence of AuNPs-AptCAP through changing the efficiency of the fluorescence resonance energy transfer (FRET) system with Cy3 as donors and AuNPs as recipients. AuNPs-AptCAP exhibits a linear range of CAP concentrations from 26.0 to 277 μg L−1 with a limit of detection of 8.1 μg L−1 when Cy3 was excited at 530 nm and emission was measured at 570 nm. More importantly, AuNPs-AptCAP can be utilized as signal transducers for the build-up of a series of logic gates including YES, PASS 0, INH, NOT, PASS 1, and NAND. Utilizing the principle of a metal ion–mediated fluorescence switch together with a strong metal ion chelator, the fluorescence of AuNPs-AptCAP could be modulated by adding metal ions and EDTA sequentially. Therefore, a “Plug and Play” logic system based on AuNPs-AptCAP has been realized by simply adding other components to create new logic functions. This work highlights the advantages of simple synthesis and facile fluorescence switching properties, which will provide useful knowledge for the establishment of molecular logic systems.

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References

  1. de Silva AP, Uchiyama S (2007) Molecular logic and computing. Nat Nanotechnol 2(7):399–410. https://doi.org/10.1038/nnano.2007.188

    Article  CAS  Google Scholar 

  2. Szacilowski K (2008) Digital information processing in molecular systems. Chem Rev 108(9):3481–3548. https://doi.org/10.1021/cr068403q

    Article  CAS  Google Scholar 

  3. Tregubov AA, Nikitin PI, Nikitin MP (2018) Advanced smart nanomaterials with integrated logic-gating and biocomputing: dawn of theranostic nanorobots. Chem Rev 118(20):10294–10348. https://doi.org/10.1021/acs.chemrev.8b00198

    Article  CAS  Google Scholar 

  4. Pischel U (2007) Chemical approaches to molecular logic elements for addition and subtraction. Angew Chem Int Ed 46(22):4026–4040. https://doi.org/10.1002/anie.200603990

    Article  CAS  Google Scholar 

  5. Pu F, Ren J, Qu X (2014) Nucleic acids and smart materials: advanced building blocks for logic systems. Adv Mater 26(33):5742–5757. https://doi.org/10.1002/adma.201401617

    Article  CAS  Google Scholar 

  6. Seelig G, Soloveichik D, Zhang DY, Winfree E (2006) Enzyme-free nucleic acid logic circuits. Science. 314(5805):1585–1588. https://doi.org/10.1126/science.1132493

    Article  CAS  Google Scholar 

  7. Liu D, Chen W, Sun K, Deng K, Zhang W, Wang Z, Jiang X (2011) Resettable, multi-readout logic gates based on controllably reversible aggregation of gold nanoparticles. Angew Chem Int Ed 50(18):4103–4107. https://doi.org/10.1002/anie.201008198

    Article  CAS  Google Scholar 

  8. Chen X, Wang YF, Liu Q, Zhang ZZ, Fan CH, He L (2006) Construction of molecular logic gates with a DNA-cleaving deoxyribozyme. Angew Chem Int Ed 45(11):1759–1762. https://doi.org/10.1002/anie.200502511

    Article  CAS  Google Scholar 

  9. Deng M, Yang M, Xu Y, Sun Y, Wang Q, Liu J, Huang J, Yang X, Wang K (2019) Biomimetic nanochannel membrane for cascade response of borate and cis-hydroxyl compounds: an IMP logic gate device. Chin Chem Lett 30(7):1397–1400. https://doi.org/10.1016/j.cclet.2019.04.003

    Article  CAS  Google Scholar 

  10. Qu DH, Wang QC, Tian H (2005) A half adder based on a photochemically driven 2 rotaxane. Angew Chem Int Ed 44(33):5296–5299. https://doi.org/10.1002/anie.200501215

    Article  CAS  Google Scholar 

  11. Langford SJ, Yann T (2003) Molecular logic: a half-subtractor based on tetraphenylporphyrin (vol 125, 11198, 2003). J Am Chem Soc 125(48):14951–14951. https://doi.org/10.1021/ja038843o

    Article  CAS  Google Scholar 

  12. Margulies D, Melman G, Shanzer A (2006) A molecular full-adder and full-subtractor, an additional step toward a moleculator. J Am Chem Soc 128(14):4865–4871. https://doi.org/10.1021/ja058564w

    Article  CAS  Google Scholar 

  13. Credi A (2007) Molecules that make decisions. Angew Chem Int Ed 46(29):5472–5475. https://doi.org/10.1002/anie.200700879

    Article  CAS  Google Scholar 

  14. Pan Y, Shi Y, Chen Z, Chen J, Hou M, Chen Z, Li C-W, Yi C (2016) Design of multiple logic gates based on chemically triggered fluorescence switching of functionalized polyethylenimine. ACS Appl Mater Interfaces 8(14):9472–9482. https://doi.org/10.1021/acsami.6b02080

    Article  CAS  Google Scholar 

  15. de Silva AP, Dobbin CM, Vancea TP, Wannalerse B (2009) Multiply reconfigurable ‘plug and play’ molecular logic via self-assembly. Chem Commun 11:1386–1388. https://doi.org/10.1039/b822181b

    Article  CAS  Google Scholar 

  16. Shanley T (1995) Plug and play system architecture

  17. Auslaender S, Auslaender D, Mueller M, Wieland M, Fussenegger M (2012) Programmable single-cell mammalian biocomputers. Nature 487:123–127. https://doi.org/10.1038/nature11149

    Article  CAS  Google Scholar 

  18. Pu F, Ren J, Qu X (2014) “Plug and Play” logic gates based on fluorescence switching regulated by self-assembly of nucleotide and lanthanide ions. ACS Appl Mater Interfaces 6(12):9557–9562. https://doi.org/10.1021/am501949t

    Article  CAS  Google Scholar 

  19. Chang X, Zhang C, Lv C, Sun Y, Zhang M, Zhao Y, Yang L, Han D, Tan W (2019) Construction of a multiple-aptamer-based DNA logic device on live cell membranes via associative toehold activation for accurate cancer cell identification. J Am Chem Soc 141(32):12738–12743. https://doi.org/10.1021/jacs.9b05470

    Article  CAS  Google Scholar 

  20. Yang J, Jiang S, Liu X, Pan L, Zhang C (2016) Aptamer-binding directed DNA origami pattern for logic gates. ACS Appl Mater Interfaces 8(49):34054–34060. https://doi.org/10.1021/acsami.6b10266

    Article  CAS  Google Scholar 

  21. Wang L, Zhu J, Han L, Jin L, Zhu C, Wang E, Dong S (2012) Graphene-based aptamer logic gates and their application to multiplex detection. ACS Nano 6(8):6659–6666. https://doi.org/10.1021/nn300997f

    Article  CAS  Google Scholar 

  22. Shukoor MI, Altman MO, Han D, Bayrac AT, Ocsoy I, Zhu Z, Tan W (2012) Aptamer-nanoparticle assembly for logic-based detection. ACS Appl Mater Interfaces 4(6):3007–3011. https://doi.org/10.1021/am300374q

    Article  CAS  Google Scholar 

  23. Zhou M, Du Y, Chen C, Li B, Wen D, Dong S, Wang E. (2010) Aptamer-controlled biofuel cells in logic systems and used as self-powered and intelligent logic aptasensors. J Am Chem Soc.132(7):2172−+. doi:https://doi.org/10.1021/ja910634e

  24. Tuerk C, Gold L (1990) Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science (New York, NY) 249(4968):505–510. https://doi.org/10.1126/science.2200121

    Article  CAS  Google Scholar 

  25. Shi Q, Shi Y, Pan Y, Yue Z, Zhang H, Yi C (2015) Colorimetric and bare eye determination of urinary methylamphetamine based on the use of aptamers and the salt-induced aggregation of unmodified gold nanoparticles (vol 182, pg 505, 2015). Microchim Acta 182(3–4):513–513. https://doi.org/10.1007/s00604-014-1396-1

    Article  CAS  Google Scholar 

  26. Kuang L, Cao S-P, Zhang L, Li Q-H, Liu Z-C, Liang R-P, Qiu J-D (2016) A novel nanosensor composed of aptamer bio-dots and gold nanoparticles for determination of thrombin with multiple signals. Biosens Bioelectron 85:798–806. https://doi.org/10.1016/j.bios.2016.05.096

    Article  CAS  Google Scholar 

  27. Li L, Liu Z, Zhang H, Yue W, Li C-W, Yi C (2018) A point-of-need enzyme linked aptamer assay for Mycobacterium tuberculosis detection using a smartphone. Sensors Actuators B Chem 254:337–346. https://doi.org/10.1016/j.snb.2017.07.074

    Article  CAS  Google Scholar 

  28. Robati RY, Arab A, Ramezani M, Langroodi FA, Abnous K, Taghdisi SM (2016) Aptasensors for quantitative detection of kanamycin. Biosens Bioelectron 82:162–172. https://doi.org/10.1016/j.bios.2016.04.011

    Article  CAS  Google Scholar 

  29. Qu H, Csordas AT, Wang J, Oh SS, Eisenstein MS, Soh HT (2016) Rapid and label-free strategy to isolate aptamers for metal ions. ACS Nano 10(8):7558–7565. https://doi.org/10.1021/acsnano.6b02558

    Article  CAS  Google Scholar 

  30. Liu Z, Zhang Y, Xu S, Zhang H, Tan Y, Ma C, Song R, Jiang L, Yi C (2017) A 3D printed smartphone optosensing platform for point-of-need food safety inspection. Anal Chim Acta 966:81–89. https://doi.org/10.1016/j.aca.2017.02.022

    Article  CAS  Google Scholar 

  31. Hu Z, Jian J, Hua Y, Yang D, Gao Y, You J, Wang Z, Chang Y, Yuan K, Bao Z, Zhang Q, Li S, Jiang Z, Zhou H (2018) DNA colorimetric logic gate in microfluidic chip based on unmodified gold nanoparticles and molecular recognition. Sensors Actuators B Chem 273:559–565. https://doi.org/10.1016/j.snb.2018.06.073

    Article  CAS  Google Scholar 

  32. Yang J, Song Z, Liu S, Zhang Q, Zhang C (2016) Dynamically arranging gold nanoparticles on DNA origami for molecular logic gates. ACS Appl Mater Interfaces 8(34):22451–22456. https://doi.org/10.1021/acsami.6b04992

    Article  CAS  Google Scholar 

  33. Li Y, Li W, He K-Y, Li P, Huang Y, Nie Z, Yao S-Z (2016) A biomimetic colorimetric logic gate system based on multi-functional peptide-mediated gold nanoparticle assembly. Nanoscale. 8(16):8591–8599. https://doi.org/10.1039/c6nr01072e

    Article  CAS  Google Scholar 

  34. de la Rosa VR, Zhang Z, De Geest BG, Hoogenboom R (2015) Colorimetric logic gates based on poly(2-alkyl-2-oxazoline)coated gold nanoparticles. Adv Funct Mater 25(17):2511–2519. https://doi.org/10.1002/adfm.201404560

    Article  CAS  Google Scholar 

  35. Meng T-T, Liu Y-X, Liu M-T, Long J-B, Cao Q-F, Yan S-Y, Meng X-X (2015) Lineal DNA logic gate for microRNA diagnostics with strand displacement and fluorescence resonance energy transfer. Chin Chem Lett 26(9):1179–1182. https://doi.org/10.1016/j.cclet.2015.05.039

    Article  CAS  Google Scholar 

  36. Su S, Sun H, Cao W, Chao J, Peng H, Zuo X, Yuwen L, Fan C, Wang L (2016) Dual-target electrochemical biosensing based on DNA structural switching on gold nanoparticle-decorated MoS2 nanosheets. ACS Appl Mater Interfaces 8(11):6826–6833. https://doi.org/10.1021/acsami.5b12833

    Article  CAS  Google Scholar 

  37. Qin C, Gao Y, Wen W, Zhang X, Wang S (2016) Visual multiple recognition of protein biomarkers based on an array of aptamer modified gold nanoparticles in biocomputing to strip biosensor logic operations. Biosens Bioelectron 79:522–530. https://doi.org/10.1016/j.bios.2015.12.096

    Article  CAS  Google Scholar 

  38. Wang J, Lu J, Su S, Gao J, Huang Q, Wang L, Huang W, Zuo X (2015) Binding-induced collapse of DNA nano-assembly for naked-eye detection of ATP with plasmonic gold nanoparticles. Biosens Bioelectron 65:171–175. https://doi.org/10.1016/j.bios.2014.10.031

    Article  CAS  Google Scholar 

  39. Zhang Y, Qi S, Liu Z, Shi Y, Yue W, Yi C (2016) Rapid determination of dopamine in human plasma using a gold nanoparticle-based dual-mode sensing system. Mater Sci Eng C 61:207–213. https://doi.org/10.1016/j.msec.2015.12.038

    Article  CAS  Google Scholar 

  40. Xu X, Zhang J, Yang F, Yang X (2011) Colorimetric logic gates for small molecules using split/integrated aptamers and unmodified gold nanoparticles. Chem Commun 47(33):9435–9437. https://doi.org/10.1039/c1cc13459k

    Article  CAS  Google Scholar 

  41. Grabar KC, Freeman RG, Hommer MB, Natan MJ (1995) Preparation and characterization of Au colloid monolayers. Anal Chem 67:735–743. https://doi.org/10.1021/ac00100a008‚

    Article  CAS  Google Scholar 

  42. Mehta J, Van Dorst B, Rouah-Martin E, Herrebout W, Scippo M-L, Blust R, Robbens J (2011) In vitro selection and characterization of DNA aptamers recognizing chloramphenicol. J Biotechnol 155(4):361–369. https://doi.org/10.1016/j.jbiotec.2011.06.043

    Article  CAS  Google Scholar 

  43. Shi Y, Zhang H, Yue Z, Zhang Z, Teng K-S, Li M-J, Yi C, Yang M (2013) Coupling gold nanoparticles to silica nanoparticles through disulfide bonds for glutathione detection. Nanotechnology. 24(37):375501. https://doi.org/10.1088/0957-4484/24/37/375501

    Article  CAS  Google Scholar 

  44. Kim Y-P, Oh Y-H, Oh E, Ko S, Han M-K, Kim H-S (2008) Energy transfer-based multiplexed assay of proteases by using gold nanoparticle and quantum dot conjugates on a surface. Anal Chem 80(12):4634–4641. https://doi.org/10.1021/ac702416e

    Article  CAS  Google Scholar 

  45. Zu F, Yan F, Bai Z, Xu J, Wang Y, Huang Y, Zhou X (2017) The quenching of the fluorescence of carbon dots: a review on mechanisms and applications. Microchim Acta 184(7):1899–1914. https://doi.org/10.1007/s00604-017-2318-9

    Article  CAS  Google Scholar 

  46. Shi Y, Yi C, Zhang Z, Zhang H, Li M, Yang M, Jiang Q (2013) Peptide-bridged assembly of hybrid nanomaterial and its application for caspase-3 detection. ACS Appl Mater Interfaces 5(14):6494–6501. https://doi.org/10.1021/am401935y

    Article  CAS  Google Scholar 

  47. Phan AT, Kuryavyi V, Patel DJ (2006) DNA architecture: from G to Z. Curr Opin Struct Biol 16(3):288–298. https://doi.org/10.1016/j.sbi.2006.05.011

    Article  CAS  Google Scholar 

  48. Meggers E, Holland PL, Tolman WB, Romesberg FE, Schultz PG (2000) A novel copper-mediated DNA base pair. J Am Chem Soc 122(43):10714–10715. https://doi.org/10.1021/ja0025806

    Article  CAS  Google Scholar 

  49. Daniel SCGK, Kumar A, Sivasakthi K, Thakur CS (2019) Handheld, low-cost electronic device for rapid, real-time fluorescence-based detection of Hg2+, using aptamer-templated ZnO quantum dots. Sensors Actuators B Chem 290:73–78. https://doi.org/10.1016/j.snb.2019.03.113

    Article  CAS  Google Scholar 

  50. Zhan S, Wu Y, Wang L, Zhan X, Zhou P (2016) A mini-review on functional nucleic acids-based heavy metal ion detection. Biosens Bioelectron 86:353–368. https://doi.org/10.1016/j.bios.2016.06.075

    Article  CAS  Google Scholar 

  51. Huang J, Su X, Li Z (2017) Metal ion detection using functional nucleic acids and nanomaterials. Biosens Bioelectron 96:127–139. https://doi.org/10.1016/j.bios.2017.04.032

    Article  CAS  Google Scholar 

  52. Demers LM, Mirkin CA, Mucic RC, Reynolds RA, Letsinger RL, Elghanian R, Viswanadham G (2000) A fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles. Anal Chem 72(22):5535–5541. https://doi.org/10.1021/ac0006627

    Article  CAS  Google Scholar 

  53. Hurst SJ, Lytton-Jean AKR, Mirkin CA (2006) Maximizing DNA loading on a range of gold nanoparticle sizes. Anal Chem 78(24):8313–8318. https://doi.org/10.1021/ac0613582

    Article  CAS  Google Scholar 

  54. Zanchet D, Micheel CM, Parak WJ, Gerion D, Alivisatos AP (2001) Electrophoretic isolation of discrete Au nanocrystal/DNA conjugates. Nano Lett 1(1):32–35. https://doi.org/10.1021/nl005508e

    Article  CAS  Google Scholar 

  55. Cao X-H, Wang Q, Li J, Yi C, Li M-J (2017) Gold nanoparticles functionalized with Ru(II)bipyridyl labeled DNA as a luminescent probe for the sensitive determination of DNase I. Microchim Acta 184(9):3273–3279. https://doi.org/10.1007/s00604-017-2330-0

    Article  CAS  Google Scholar 

  56. Rose PW, Hamden A, Brueggemann AB, Perera R, Sheikh A, Crook D, Mant D (2005) Chloramphenicol treatment for,acute infective conjunctivitis in children in primary care: a randomised double-blind placebo-controlled trial. Lancet. 366(9479):37–43. https://doi.org/10.1016/s0140-6736(05)66709-8

    Article  CAS  Google Scholar 

  57. Suarez CR, Ow EP (1992) Chloramphenicol toxicity associated with severe cardiac dysfunction. Pediatr Cardiol 13(1):48–51

    CAS  Google Scholar 

  58. No. 235 of the Ministry of Agriculture of the People's Republic of China, 2002, http://jiuban.moa.gov.cn/zwllm/ tzgg/gg/200302/P0200506

  59. Commission Decision of 13 (2003) http://data.europa.eu/eli/dec/2003/181(1)/oj

  60. Giri AS, Golder AK (2014) Chloramphenicol degradation in Fenton and photo-Fenton: formation of Fe2+-chloramphenicol chelate and reaction pathways. Ind Eng Chem Res 53(42):16196–16203. https://doi.org/10.1021/ie501508d

    Article  CAS  Google Scholar 

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Funding

The financial support from Guangdong Natural Science Foundation (S2017A030313076), Shenzhen Basic Research Program (JCYJ20170307140752183), and Science and Technology Project of Guangzhou (201803020026) is received.

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  1. Yali Zhang and Cheuk-Wing Li contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Sensing Technology and Biomedical Instruments (Guangdong Province), School of Biomedical Engineering, Sun Yat-Sen University, Guangzhou, 510006, China

    Yali Zhang, Lefei Zhou, Zhanpeng Chen & Changqing Yi

  2. School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham, NG11 8NS, UK

    Cheuk-Wing Li

  3. Research Institute of Sun Yat-Sen University in Shenzhen, Shenzhen, 518057, China

    Changqing Yi

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  1. Yali Zhang
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Zhang, Y., Li, CW., Zhou, L. et al. “Plug and Play” logic gate construction based on chemically triggered fluorescence switching of gold nanoparticles conjugated with Cy3-tagged aptamer. Microchim Acta 187, 437 (2020). https://doi.org/10.1007/s00604-020-04421-5

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