Phase diagrams are widely used to map and control the assembly states of biopolymers. However, these studies are highly time and material consuming. Here, we present the optimization of a simple tool based on millifluidics to screen phase diagram of biopolymers, pure or in mixtures with other biopolymers. It is used (i) to generate a homogeneous mixture of biopolymers/buffer and/or biopolymers 1/ biopolymers 2 in a short time (~s), (ii) to vary the composition of the mixtures by adjusting the flow rates, and (iii) to determine the turbidity of the drop by grey level analysis. The mixing efficiency and the calibration turbidity vs. grey level were performed using colloidal titanium dioxide dispersions. The use of millifluidics reduced the amount of material of ten-fold and the experimentation time by a factor five compared to a conventional bulk approach. This set-up was used to explore functional synergies between proteins and polysaccharides as an example of application.

相图被广泛用于绘制和控制生物聚合物的组装状态。但是，这些研究非常耗时且耗材。在这里，我们介绍了一种基于微流控技术的简单工具的优化，以筛选纯生物聚合物或与其他生物聚合物混合的生物聚合物的相图。它可用于（i）在短时间内（〜s）生成生物聚合物/缓冲液和/或生物聚合物1 /生物聚合物2的均质混合物，（ii）通过调节流速来改变混合物的组成，以及（ iii）通过灰度分析确定液滴的浊度。使用胶态二氧化钛分散体进行混合效率和校准浊度对灰度的比较。与传统的批量方法相比，使用微流体技术可将材料数量减少十倍，并将实验时间减少五倍。此应用程序被用来探索蛋白质和多糖之间的功能协同作用，作为应用实例。