Abstract

A method is proposed for the determination of cysteine in an aqueous solution by its effect on the formation of gold nanoparticles on the surface of polyurethane foams. The method is based on the interaction of in situ formed gold nanoparticles with cysteine, which results in their stabilization in solution and in a decrease in the color intensity of the nanocomposite detected by diffuse reflectance spectroscopy. The effect of the concentration of the precursor, aurichlorohydric acid, contact time of phases, and the pH of the solution on the sensitivity of cysteine determination is studied. Under the selected conditions, the limit of detection for cysteine was 0.36 μg/mL. A possibility of using the proposed method for the determination of cysteine in brewer’s yeast is demonstrated.

中文翻译：

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半胱氨酸对聚氨酯泡沫金纳米复合材料形成的影响，通过漫反射光谱法测定

摘要

提出了一种测定水溶液中半胱氨酸的方法，该方法对聚氨酯泡沫表面金纳米颗粒的形成有影响。该方法基于原位形成的金纳米颗粒与半胱氨酸的相互作用，这导致它们在溶液中稳定并且通过漫反射光谱法检测到的纳米复合材料的色强度降低。研究了前体浓度，金氯氢酸，各相的接触时间和溶液的pH对半胱氨酸测定灵敏度的影响。在所选条件下，半胱氨酸的检出限为0.36μg/ mL。证明了使用所提出的方法测定啤酒酵母中半胱氨酸的可能性。