Abstract

The effects of medium viscosity on the decay rate of the 4a-hydroperoxyflavin intermediate of the bioluminescent reaction was investigated. It was found that at low concentrations of glycerol or sucrose (viscosity 1.1–1.3 cP) the decay rate rises, whereas a further increase in viscosity to 6.2 cP leads to a decrease in the decay rate following a power function with an exponent of 0.82–0.84. Using molecular dynamics methods, it was shown that the presence of glycerol and sucrose molecules causes a change in the mobility of the amino acid residues in the active center of luciferase, particularly those responsible for binding of flavin. The results obtained are indicative of two opposite effects of viscous media with glycerol and sucrose: (1) destabilization of 4a-hydroperoxyflavin due to a change in the structural and dynamic properties of the protein and (2) stabilization of this intermediate by the decrease in the diffusion rate of its decay products.

中文翻译：

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粘性介质会减慢细菌生物发光反应中关键中间体的衰减。

摘要

研究了介质粘度对生物发光反应中4a-氢过氧黄素中间体降解速率的影响。研究发现，在低浓度的甘油或蔗糖（粘度为1.1–1.3 cP）下，衰减率会提高，而粘度进一步提高至6.2 cP时，幂函数为0.82–2时，衰减率会下降。 0.84。使用分子动力学方法显示，甘油和蔗糖分子的存在引起萤光素酶活性中心的氨基酸残基，特别是负责黄素结合的氨基酸残基的迁移率发生变化。获得的结果表明粘性介质与甘油和蔗糖的两种相反作用：