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Viscous Media Slow Down the Decay of the Key Intermediate in Bacterial Bioluminescent Reaction

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

The effects of medium viscosity on the decay rate of the 4a-hydroperoxyflavin intermediate of the bioluminescent reaction was investigated. It was found that at low concentrations of glycerol or sucrose (viscosity 1.1–1.3 cP) the decay rate rises, whereas a further increase in viscosity to 6.2 cP leads to a decrease in the decay rate following a power function with an exponent of 0.82–0.84. Using molecular dynamics methods, it was shown that the presence of glycerol and sucrose molecules causes a change in the mobility of the amino acid residues in the active center of luciferase, particularly those responsible for binding of flavin. The results obtained are indicative of two opposite effects of viscous media with glycerol and sucrose: (1) destabilization of 4a-hydroperoxyflavin due to a change in the structural and dynamic properties of the protein and (2) stabilization of this intermediate by the decrease in the diffusion rate of its decay products.

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Funding

This work was supported by the Ministry of Science and Higher Education of the Russian Federation (project nos. 6.7734.2017 and 01201351504).

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Authors and Affiliations

  1. Siberian Federal University, Krasnoyarsk, Russia

    A. E. Lisitsa, L. A. Sukovatyi, V. A. Kratasyuk & E. V. Nemtseva

  2. Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russia

    V. A. Kratasyuk & E. V. Nemtseva

Authors
  1. A. E. Lisitsa
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  2. L. A. Sukovatyi
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  3. V. A. Kratasyuk
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  4. E. V. Nemtseva
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Correspondence to A. E. Lisitsa.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by M. Batrukova

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Lisitsa, A.E., Sukovatyi, L.A., Kratasyuk, V.A. et al. Viscous Media Slow Down the Decay of the Key Intermediate in Bacterial Bioluminescent Reaction. Dokl Biochem Biophys 492, 162–165 (2020). https://doi.org/10.1134/S1607672920020106

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  • DOI: https://doi.org/10.1134/S1607672920020106

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