The purpose of this study was to determine the biogas potential of biomass produced by microbiotic communities developed under natural conditions in freshwater systems such as ponds incorporated into agricultural landscapes. Natural communities of microalgae were collected from a small eutrophicated pond where dominant species were euglenoids (Lepocinclis species). Cyanobacterial communities dominated by Lyngbya species were taken from a domestic aquarium and cultivated under makeshift conditions. Experiments were done using dairy cow manure (DCM) for codigestion with natural communities of microalgae (MDM) and cyanobacteria (CDM) and conducted during 42 days in thermophilic regime. The total biogas yields were 421.40 and 383.34 mL/g volatile solids (VS), while the average methane contents were 63.97 and 64.06% for MDM and CDM, respectively. Our results indicate that the natural communities of microalgae and cyanobacteria used in this study possess the potential for biogas production, which is, in comparison with particular algal and cyanobacterial strains cultivated under strictly controlled cultivation conditions, more promising. Therefore, this study aims to motivate further investigations into the diverse natural communities of microalgae and cyanobacteria and pretreatments that are environmentally friendly and cost-effective and will eventually enhance small-scale biogas production on agricultural farms.

这项研究的目的是确定在自然条件下在淡水系统（例如纳入农业景观的池塘）中发展的微生物群落所产生的生物质的沼气潜力。微藻的自然群落是从一个富营养化的小池塘中收集的，那里的主要物种是类鳗鱼（Lepocinclis物种）。Lyngbya主导的蓝细菌群落该物种取自家用水族馆，并在临时条件下养殖。使用奶牛粪便（DCM）与微藻（MDM）和蓝藻（CDM）的自然群落共消化进行了实验，并在嗜热条件下进行了42天。总沼气产量为421.40和383.34 mL / g挥发性固体（VS），而MDM和CDM的平均甲烷含量分别为63.97和64.06％。我们的结果表明，本研究中使用的微藻和蓝细菌的自然群落具有生产沼气的潜力，与在严格控制的栽培条件下种植的特定藻类和蓝细菌菌株相比，这更有希望。因此，