Dryopteris crassirhizoma dryocrassin ABBA is a kind of phloroglucinol compound isolated from the whole plants of Dryopteris crassirhizoma. While previous studies have shown that dryocrassin ABBA induces various defence responses in potato tubers, limited information is known about the mechanism of dryocrassin ABBA-induced plant responses. The transcriptome profiles in potato tubers after treatment with dryocrassin ABBA or distilled water were analysed using RNA-Seq. The profiling analysis revealed a total of 37 million clean reads and 3252 differentially expressed genes (DEGs). The data were further confirmed by real-time PCR. The results suggest that dryocrassin ABBA treatment enhances resistance in potatoes, which may be primarily mediated by the salicylic acid (SA) signal pathway, while the jasmonic acid (JA), the ethylene (ET) and the abscisic acid (ABA) signal pathways effectively induce resistance in potatoes. Moreover, the transcripts related to flavonoid metabolism were also up-regulated in potatoes after dryocrassin ABBA treatment, demonstrating that secondary metabolites are related to the constitutive activation of the defence response in dryocrassin ABBA-treated potatoes. These findings reveal that the induced resistance of dryocrassin ABBA is related tomultiple signaling pathways and defence components to enhance host defence responses in potato tubers.

中文翻译：

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马铃薯块茎Dryopteris crassirhizoma Dryocrassin ABBA接种的抗性的转录组谱分析

鳞翅目干枯菌ABOC是从鳞翅目枯草整个植物中分离得到的一种间苯三酚化合物。虽然以前的研究表明，干球蛋白ABBA可以诱导马铃薯块茎产生各种防御反应，但关于干球蛋白ABBA诱导植物反应的机制的信息知之甚少。使用RNA-Seq分析了干球蛋白ABBA或蒸馏水处理后马铃薯块茎的转录组谱。分析分析显示，总共有3,700万个纯读和3252个差异表达基因（DEG）。数据通过实时PCR进一步证实。结果表明，干球蛋白ABBA处理可增强马铃薯的抗性，这可能主要由水杨酸（SA）信号途径介导，而茉莉酸（JA），乙烯（ET）和脱落酸（ABA）信号途径有效诱导马铃薯产生抗药性。此外，在干球蛋白ABBA处理后，马铃薯中与类黄酮代谢有关的转录本也上调，表明次级代谢产物与在用干球蛋白ABBA处理的马铃薯中防御反应的组成性激活有关。这些发现表明，干球蛋白ABBA的诱导抗性与多种信号途径和防御组分相关，以增强马铃薯块茎中的宿主防御反应。