Bacterial blight is a common disease found in the rice-growing regions in the Korean peninsula. Identification of the gene network involved against Xanthomonas oryzae pv. oryzae Korean race K2 in popular japonica cultivars is essential in underpinning the molecular mechanism of resistance. A microarray of two popular Korean japonica rice cultivars, a bacterial blight susceptible Dongjin and resistant Jinbaek, was performed to investigate the transcripts of inducible genes at 48 h post-inoculation. A total of 771 differentially expressed genes were identified in Jinbaek, whereas 298 were found in Dongjin. The resistance observed in Jinbaek is likely participated by genes with predicted functions in transmembrane perception, intracellular signal transduction, and transcription activity. Moreover, the remarkable involvement of numerous WRKY proteins signifies orchestration of defense signals via robust cellular reprogramming, which leads to resistance. To discover genes essential to bacterial blight resistance in Jinbaek, 13 highly expressed genes encoding different protein classes were cloned and overexpressed in rice. Although none of the overexpression plants exhibited resistance comparable to Jinbaek, four candidate genes, including one twin-arginine translocation pathway signal (LOC_Os01g45640.1), one cytochrome p450 (LOC_Os09g10340.1), and two uncharacterized expressed protein (LOC_Os08g26230.4, LOC_Os09g04310.1) conferred partial resistance. However, of these four genes, only p450s have been reported to play an important role in the synthesis of plant defense compounds. These findings revealed the complexity of key immune signaling conduits critical to mounting a full defense against Xanthomonas. oryzae pv. oryzae race K2 in japonica rice.

中文翻译：

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对水稻黄单胞菌抗性的转录调节。粳稻稻米韩国人K2种族

细菌性疫病是朝鲜半岛水稻种植地区常见的疾病。鉴定抗稻瘟病单胞菌PV的基因网络。流行的粳稻品种中的稻米朝鲜族K2对增强抗性的分子机制至关重要。两个流行的韩国粳稻的微阵列水稻品种，一种细菌性枯萎病易感的东晋和抗性金百克，进行了调查，以调查接种后48 h诱导基因的转录本。在金柏市共鉴定了771个差异表达基因，而在东金市则发现了298个差异表达基因。在Jinbaek中观察到的抗性可能与在跨膜感知，细胞内信号转导和转录活性中具有预测功能的基因有关。此外，大量WRKY蛋白的显着参与标志着通过强大的细胞重编程来协调防御信号，从而导致耐药性。为了发现金白细菌抗白叶枯病必不可少的基因，在水稻中克隆并过表达了13种高表达基因，它们编码不同的蛋白质类型。尽管没有一种过表达植物显示出与Jinbaek相当的抗性，但有四个候选基因，包括一个双精氨酸易位途径信号（LOC_Os01g45640.1），一个细胞色素p450（LOC_Os09g10340.1）和两个未表征的表达蛋白（LOC_Os08g26230.4，LOC_Os09g04310 .1）赋予部分抵抗力。但是，在这四个基因中，据报道只有p450在植物防御化合物的合成中起重要作用。这些发现揭示了关键的免疫信号传导导管的复杂性，这对于全面防御 然而，在这四个基因中，据报道只有p450在植物防御化合物的合成中起重要作用。这些发现揭示了关键的免疫信号传导导管的复杂性，这对于全面防御 然而，在这四个基因中，据报道只有p450在植物防御化合物的合成中起重要作用。这些发现揭示了关键的免疫信号传导导管的复杂性，这对于全面防御黄单胞菌。菌PV。粳稻中的Oryzae种族K2。