Leptocarpha rivularis, commonly known as “palo negro”, is a species of the Astereaceae family, endemic in the forests of Southern Chile. The extracts of this plant have medicinal properties attributable to its secondary metabolites, mainly identified as flavonoids and terpenes. Among them, leptocarpine has gained importance as a powerful anticarcinogen. The aim of this study was the in vitro establishment of L. rivularis to develop future alternatives to obtain biomass for medicinal purposes. Young shoots obtained from plants in growth chambers were treated with Captan™ (2.5% (w/v)), subjected to disinfection method with ethanol, commercial chlorine, and antioxidants and tested in different Murashige & Skoog based media to establish in vitro culture. The most effective growth was observed with half strength Murashige & Skoog medium supplemented with 20 g L⁻¹ sucrose and 3.2 g L⁻¹ gelrite (MGS medium) with 95% survival at 30-d and shooting reaching 82% of the explants when Plant Preservative Mixture™ is added to the medium. The addition of 1 mg L⁻¹ of indole-3-butyric acid to the MSG medium was necessary for inducing roots and obtaining well-rooted plants that were acclimatized and successfully established in the greenhouse. Our study represents the first report describing the in vitro culture of L. rivularis, allowing the sustainable use of this plant.

近江牡蛎Leptocarpha，俗称“帕洛内格罗”，是一个物种的Astereaceae家庭，在智利南部的森林流行。该植物的提取物具有可归因于其次生代谢产物的药用特性，主要鉴定为类黄酮和萜烯。其中，钩藤碱已成为一种强大的抗癌物质。这项研究的目的是在体外建立小叶柳桉（L. rivularis），以发展未来的替代途径，以获得药用生物量。用Captan™（2.5％（w / v）处理从生长室中的植物获得的幼芽），然后用乙醇，市售氯和抗氧化剂进行消毒，并在不同的Murashige＆Skoog基培养基中进行测试以建立体外培养。在补充有20 g L ^-1蔗糖和3.2 g L ^-1胶体的半强度Murashige＆Skoog培养基（MGS培养基）中观察到最有效的生长，其在30天时的存活率为95％，在种植时射击达到外植体的82％将防腐剂Mixture™添加到培养基中。向MSG培养基中添加1 mg L ^-1的吲哚-3-丁酸对于诱导根部和获得能在温室中驯化并成功建立的有根的植物是必要的。我们的研究代表了描述体外实验的第一份报告L. rivularis的文化，使这种植物得以可持续利用。