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In vitro propagation of Leptocarpha rivularis, a native medicinal plant

  • Medicinal Plants
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Abstract

Leptocarpha rivularis, commonly known as “palo negro”, is a species of the Astereaceae family, endemic in the forests of Southern Chile. The extracts of this plant have medicinal properties attributable to its secondary metabolites, mainly identified as flavonoids and terpenes. Among them, leptocarpine has gained importance as a powerful anticarcinogen. The aim of this study was the in vitro establishment of L. rivularis to develop future alternatives to obtain biomass for medicinal purposes. Young shoots obtained from plants in growth chambers were treated with Captan™ (2.5% (w/v)), subjected to disinfection method with ethanol, commercial chlorine, and antioxidants and tested in different Murashige & Skoog based media to establish in vitro culture. The most effective growth was observed with half strength Murashige & Skoog medium supplemented with 20 g L−1 sucrose and 3.2 g L−1 gelrite (MGS medium) with 95% survival at 30-d and shooting reaching 82% of the explants when Plant Preservative Mixture™ is added to the medium. The addition of 1 mg L−1 of indole-3-butyric acid to the MSG medium was necessary for inducing roots and obtaining well-rooted plants that were acclimatized and successfully established in the greenhouse. Our study represents the first report describing the in vitro culture of L. rivularis, allowing the sustainable use of this plant.

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Acknowledgments

The authors thank to Botanical Garden of Viña del Mar and the forest engineer Patricio Novoa for their help in identifying the plants of L. rivularis, Centro de Biotecnología “Dr. Daniel Alkalay Lowitt” of Universidad Técnica Federico Santa María, the Vicerrectoría de Investigación of Universidad de Valparaíso and Centro de Investigaciones Biomédicas (CIB) of Universidad de Valparaíso.

Funding

This research was funded by FONDECYT 11160509 (I.M.) and CONICYT Programa de Investigación Asociativa (PIA) Anillo GAMBIO ACT172128 (M.S.) grants.

Author information

Author notes

  1. Fernando Dorta and Victor Polanco contributed equally to this work.

Authors and Affiliations

  1. Centro de Biotecnología “Dr. Daniel Alkalay Lowitt”, Universidad Técnica Federico Santa María, Avda. España 1680, 2390123, Valparaíso, Chile

    Fernando Dorta, Ingrid Ramírez & Michael Seeger

  2. Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Camino la Pirámide 5750, 8580000, Santiago, Chile

    Victor Polanco

  3. Laboratorio de Microbiología Molecular y Biotecnología Ambiental. Departamento de Química, Universidad Técnica Federico Santa María, Avda. España 1680, 2390123, Valparaíso, Chile

    Michael Seeger

  4. Laboratorio de Productos Naturales y Síntesis Orgánica (LPNSO), Departamento de Química, Facultad de Ciencias Naturales y Exactas, Universidad de Playa Ancha, Avda. Leopoldo Carvallo 270, Playa Ancha, 2340000, Valparaíso, Chile

    Alejandro Madrid

  5. Escuela de Obstetricia y Puericultura, Facultad de medicina, Universidad de Valparaíso, Angamos 655, Reñaca, 2520000, Viña del Mar, Chile

    Iván Montenegro

Authors
  1. Fernando Dorta
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  2. Victor Polanco
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  3. Ingrid Ramírez
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  4. Michael Seeger
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  5. Alejandro Madrid
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  6. Iván Montenegro
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Contributions

I.R. supervised the whole study. I.M. performed isolation of native plant. F.D. and V.P. conceived and designed the biologic experiments. F.D. performed the in vitro experiments. A.M. conceived and designed statistical methodologies. M.S., A.M., V.P., and I.M. collaborated in the discussion and interpretation of the results. I.M., M.S., I.R., V.P., and F.D. wrote the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Iván Montenegro.

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The authors declare that they have no conflict of interest.

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Editor: Marie-Anne Lelu-Walter

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Dorta, F., Polanco, V., Ramírez, I. et al. In vitro propagation of Leptocarpha rivularis, a native medicinal plant. In Vitro Cell.Dev.Biol.-Plant 56, 827–832 (2020). https://doi.org/10.1007/s11627-020-10097-6

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  • DOI: https://doi.org/10.1007/s11627-020-10097-6

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