BACKGROUND:Glioblastoma is the most common and deadly cancer type in the central nervous system. Following the conventional treatments in these patients, the prognosis remains poor due to high tumor recurrence. Anthocyanins from natural sources, such as blackberries (Rubus spp.), have been demonstrated an anti-proliferative effect on glioma cell lines. However, anthocyanins present in wild blackberries have been poorly studied on these cancer cells. OBJECTIVE:We aimed to determine the anthocyanins profile of two species of wild Mexican blackberries (R. liebmannii and R. palmeri), their anti-oxidant, and anti-proliferative capacities on two gliomas (C6 and RG2) cell lines. RESULTS:We concentrated the anthocyanin content at least 26 times, and revealed different proportions of these compounds in the wild blackberries. In vitro, these fractions showed anti-oxidant capacity (>10 times), and diminished cell viability (>50%) of both Rubus fractions assayed on C6 and RG2 cells (compared to control). Also, we observed increased levels of lipid peroxidation (∼59%) of malonyl dialdehyde formation in these glioma cells. Results from the cell cycle and flow cytometry assays show that anthocyanins enriched fractions elicit apoptotic responses in these glioma cells. CONCLUSIONS:Anthocyanins present in Mexican wild blackberries represent potential tools to develop alternative therapies to improve the survival rate in glioma patients.

中文翻译：

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来自两个墨西哥野生黑莓（Rubus spp。）的花青素富集部分对HepG2和神经胶质瘤细胞系的抗氧化和抗增殖作用

背景：成胶质细胞瘤是中枢神经系统中最常见和致命的癌症类型。在这些患者中进行常规治疗后，由于高肿瘤复发，预后仍然很差。来自自然来源的花青素，例如黑莓（Rubus spp。），已被证明对神经胶质瘤细胞系具有抗增殖作用。但是，野生黑莓中存在的花色苷对这些癌细胞的研究很少。目的：我们的目的是确定两种野生墨西哥黑莓（R. liebmannii和R. palmeri）的花色素苷谱，其抗氧化剂和对两种神经胶质瘤（C6和RG2）细胞系的抗增殖能力。结果：我们将花色苷含量至少浓缩了26倍，并在野生黑莓中发现了这些化合物的不同比例。体外，这些级分显示出抗氧化能力（> 10倍），并且在C6和RG2细胞上测定的两种Rubus级分的细胞活力均降低（> 50％）（与对照相比）。此外，我们观察到这些神经胶质瘤细胞中丙二酰二醛形成的脂质过氧化水平（〜59％）增加。细胞周期和流式细胞仪测定的结果表明，富含花青素的组分在这些神经胶质瘤细胞中引起凋亡反应。结论：墨西哥野生黑莓中存在的花青素是开发替代疗法以提高神经胶质瘤患者生存率的潜在工具。我们观察到这些神经胶质瘤细胞中丙二酰二醛形成的脂质过氧化水平（〜59％）增加。细胞周期和流式细胞仪测定的结果表明，富含花青素的组分在这些神经胶质瘤细胞中引起凋亡反应。结论：墨西哥野生黑莓中存在的花青素是开发替代疗法以提高神经胶质瘤患者生存率的潜在工具。我们观察到这些神经胶质瘤细胞中丙二酰二醛形成的脂质过氧化水平（〜59％）增加。细胞周期和流式细胞仪测定的结果表明，富含花青素的组分在这些神经胶质瘤细胞中引起凋亡反应。结论：墨西哥野生黑莓中存在的花青素是开发替代疗法以提高神经胶质瘤患者生存率的潜在工具。