Enhancing both the humoral and cellular immune response for tumor vaccination remains a challenge. Inspired by natural pathogen structures, we took β-glucan particles derived from a baker's yeast cell shell (YS) as a vaccine carrier and danger signal for dendritic cells (DCs), and coated the YS with catanionic layered double hydroxides (LDH) by electrostatic adsorption to form a biomimetic yeast cell particle (YSL). Our experimental results showed that the YSL vaccine efficiently targeted antigen-presenting cells (APCs) and remarkably enhanced antigen cross-presentation, and strongly improved the activation and maturation of DCs. Moreover, the YSL vaccine elicited an extremely high antibody titer and strong antigen-specific cytotoxic T lymphocyte together with mixed Th1/Th17 cellular immune responses and induced marked prophylactic and therapeutic effects against E.G7-OVA tumors in mouse models. These results suggest that YSL, integrating a yeast shell to mimic natural pathogens and LDH with high antigen-loading capacity and lysosome escape, is a promising tumor vaccine platform for rapid, effective and strong induction of both humoral and cellular immune responses.

中文翻译：

---

一种涂有层状双氢氧化物的仿生酵母壳疫苗可诱导针对肿瘤的强大体液和细胞免疫反应

增强肿瘤疫苗接种的体液和细胞免疫反应仍然是一个挑战。受自然病原体结构的启发，我们将来自面包酵母细胞壳（YS）的β-葡聚糖颗粒作为疫苗载体和树突状细胞（DCs）的危险信号，并通过静电将阳离子层状双氢氧化物（LDH）包覆在YS上。吸附形成仿生酵母细胞颗粒（YSL）。我们的实验结果表明，YSL疫苗有效靶向抗原呈递细胞（APCs）并显着增强抗原交叉呈递，并大大提高了DCs的活化和成熟。而且，YSL 疫苗在小鼠模型中引发了极高的抗体滴度和强烈的抗原特异性细胞毒性 T 淋巴细胞以及混合的 Th1/Th17 细胞免疫反应，并诱导了对 E.G7-OVA 肿瘤的显着预防和治疗作用。这些结果表明，YSL 整合了酵母外壳来模拟天然病原体和具有高抗原负载能力和溶酶体逃逸的 LDH，是一种有前途的肿瘤疫苗平台，可用于快速、有效和强烈地诱导体液和细胞免疫反应。