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Development and characterization of 14 microsatellites for the eastern chipmunk, Tamias striatus.
Molecular Biology Reports ( IF 2.6 ) Pub Date : 2020-06-25 , DOI: 10.1007/s11033-020-05586-1
Katherine Vandal 1 , Carolyne Houle 2 , Annie Archambault 3 , Denis Réale 1 , Dany Garant 2
Affiliation  

The number of microsatellite markers currently available for the eastern chipmunk Tamias striatus provides limited capacity to achieve sufficient pedigree building for the study of their genetic structure and relatedness patterns. We developed microsatellite loci for the eastern chipmunk, a small rodent commonly found in eastern North America. More specifically, we report data for 14 loci and 50 individuals genotyped from a population in southern Québec, Canada. We found a number of alleles ranging from 5 to 21 and there was no linkage disequilibrium among locus. One locus deviated from Hardy–Weinberg equilibrium and had a high proportion of null alleles. Those loci will be used in addition to previously developed loci to improve the precision of parentage assignment and population genetics studies on this species.



中文翻译:

东部花栗鼠 Tamias striatus 的 14 个微卫星的开发和表征。

目前可用于东部花栗鼠Tamias striatus的微卫星标记数量提供有限的能力来实现足够的谱系构建以研究其遗传结构和相关性模式。我们为东部花栗鼠开发了微卫星位点,东部花栗鼠是一种常见于北美东部的小型啮齿动物。更具体地说,我们报告了来自加拿大魁北克南部人口的 14 个位点和 50 个基因分型的数据。我们发现了从 5 到 21 不等的多个等位基因,并且基因座之间没有连锁不平衡。一个基因座偏离了 Hardy-Weinberg 平衡,并且具有高比例的无效等位基因。除了先前开发的基因座之外,这些基因座还将被使用,以提高该物种的亲子鉴定和种群遗传学研究的准确性。

更新日期:2020-06-26
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