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Development and characterization of 14 microsatellites for the eastern chipmunk, Tamias striatus

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Abstract

The number of microsatellite markers currently available for the eastern chipmunk Tamias striatus provides limited capacity to achieve sufficient pedigree building for the study of their genetic structure and relatedness patterns. We developed microsatellite loci for the eastern chipmunk, a small rodent commonly found in eastern North America. More specifically, we report data for 14 loci and 50 individuals genotyped from a population in southern Québec, Canada. We found a number of alleles ranging from 5 to 21 and there was no linkage disequilibrium among locus. One locus deviated from Hardy–Weinberg equilibrium and had a high proportion of null alleles. Those loci will be used in addition to previously developed loci to improve the precision of parentage assignment and population genetics studies on this species.

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Data availability

The primer sequences described here are accessible via GenBank accession numbers MN958104 to MN958117.

Code availability

Code can be provided on request.

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Acknowledgements

The work was supported by the Fonds du Québec—Nature et Technologies (FRQNT). We thank the Québec Center for Biodiversity Science (QCBS), Patrick Bergeron and Hélène Presseault-Gauvin for their help with this project. We are grateful to all graduate students and field assistants for helping to collect samples.

Funding

This study was funded by the Fonds du Québec—Nature et Technologies (FRQNT), Fund Number 148193.

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Authors and Affiliations

  1. Département des Sciences Biologiques, Université du Québec à Montréal, Montreal, QC, Canada

    Katherine Vandal & Denis Réale

  2. Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada

    Carolyne Houle & Dany Garant

  3. Céréla Inc, St-Hugues, QC, Canada

    Annie Archambault

Authors
  1. Katherine Vandal
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  2. Carolyne Houle
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  3. Annie Archambault
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  4. Denis Réale
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  5. Dany Garant
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Contributions

The study was conceived by DG and DR. The experiments were conducted by AA. The data was analyzed by KV, CH and AA. KV wrote the manuscript. All authors revised the manuscript and gave their final approval to the submitted manuscript.

Corresponding author

Correspondence to Katherine Vandal.

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The authors declare that they have no conflict of interest.

Ethics approval

All procedures performed were in accordance with the ethical standards of the institution at which the studies were conducted and ethical approval was obtained from the Université de Sherbrooke Faculty committee for animal protection (CFPA, protocol number DG2015-02-Université de Sherbrooke).

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Vandal, K., Houle, C., Archambault, A. et al. Development and characterization of 14 microsatellites for the eastern chipmunk, Tamias striatus. Mol Biol Rep 47, 6393–6397 (2020). https://doi.org/10.1007/s11033-020-05586-1

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  • DOI: https://doi.org/10.1007/s11033-020-05586-1

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