Numerous benefits related with adequate selenium supply are well-documented in all life domains; the most prominent comprise Se participation in antioxidant defense, chemopreventive activity, and alleviation of heavy metal stress. For higher plants both, favorable and toxic effects were reported, depending on the biological species, total Se concentration and on the variety/distribution of its physicochemical forms. Extensive studies focusing Se pathways and Se impact in plants have been carried out, providing data of high relevance for human health and nutrition as well as in the areas of agriculture, biotechnology, and phytoremediation. The influence of Se has been observed in all “omics” components; among them, metabolomics seems particularly interesting because the products of plant metabolism directly reflect its actual state and closely denote molecular phenotype. It should be emphasized that plant metabolites derived from Se exposure are of interest in clinical applications, in the production of dietary supplements and functional foods. In this review, recent advances in metabolomics studies of Se in higher plants are presented, primarily focusing on analytical methodology and briefly summarizing biological relevance of the obtained results. In the first part, principal research frameworks related with Se-exposed plants are presented and metabolomic approaches used in these studies are introduced. Selenium uptake and biotransformation processes are briefed, emphasizing the complexity of biological pathways and large variety of Se metabolites; analytical speciation schemes are described and located within targeted or semi-targeted approaches. Next, metabolomic analysis of plants affected by Se exposure is addressed; specific attention is given to metabolites related with stress response, and to products of primary and secondary metabolism (amino acids, fatty acids, and their oxidation products, antioxidants, glucosinolates, phenolic compounds, etc.). Finally, few examples of untargeted metabolomics are presented, highlighting multifaceted Se impact in plants. At the end, short conclusions and future prospective are furnished.

在所有生命领域，都有充分的硒供应相关的众多好处；最突出的是硒参与抗氧化防御，化学预防活性和减轻重金属胁迫。对于高等植物，都报告了有利和毒性作用，这取决于生物种类，总硒浓度及其理化形式的变化/分布。已经开展了针对硒途径和硒对植物的影响的广泛研究，提供了与人类健康和营养以及农业，生物技术和植物修复领域高度相关的数据。在所有“组学”组件中都观察到了硒的影响。其中，代谢组学似乎特别有趣，因为植物新陈代谢的产物直接反映了它的实际状态并紧密表示分子表型。应该强调的是，源自硒暴露的植物代谢物在临床应用中，在膳食补充剂和功能性食品的生产中是有意义的。在这篇综述中，介绍了高等植物中硒的代谢组学研究的最新进展，主要侧重于分析方法并简要总结了所得结果的生物学相关性。在第一部分中，介绍了与硒暴露植物相关的主要研究框架，并介绍了这些研究中使用的代谢组学方法。简要介绍了硒的吸收和生物转化过程，强调了生物途径的复杂性和多种硒代谢物；描述性物种形成方案已被描述，并位于目标或半目标方法内。接下来，对受硒暴露影响的植物进行代谢组学分析。特别注意与应激反应有关的代谢物，以及一级和二级代谢产物（氨基酸，脂肪酸及其氧化产物，抗氧化剂，芥子油苷，酚类化合物等）。最后，介绍了一些非靶向代谢组学的例子，突出了硒对植物的多方面影响。最后，提供了简短的结论和未来的展望。以及初级和次级代谢产物（氨基酸，脂肪酸及其氧化产物，抗氧化剂，芥子油苷，酚类化合物等）。最后，介绍了一些非靶向代谢组学的例子，突出了硒对植物的多方面影响。最后，提供了简短的结论和未来的展望。以及初级和次级代谢产物（氨基酸，脂肪酸及其氧化产物，抗氧化剂，芥子油苷，酚类化合物等）。最后，介绍了一些非靶向代谢组学的例子，突出了硒对植物的多方面影响。最后，提供了简短的结论和未来的展望。