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Supercritical CO2 extraction of V. vinifera leaves: Influence of cosolvents and particle size on removal kinetics and selectivity to target compounds
The Journal of Supercritical Fluids ( IF 3.9 ) Pub Date : 2020-06-25 , DOI: 10.1016/j.supflu.2020.104959
Marcelo M.R. de Melo , Benedict Carius , Mário M.Q. Simões , Inês Portugal , Jorge Saraiva , Carlos M. Silva

Leaves from Vitis vinifera L. contain potential bioactive compounds, namely: long chain aliphatic alcohols (e.g., 1-hexacosanol, 1-octacosanol and 1-triacontanol), α-tocopherol, β-sitosterol, and the triterpenes β-amyrin and lupeol. Supercritical fluid extraction (SFE) runs were measured at lab scale using: crushed and ground biomass; pure CO2 at 300 bar and 40−80 °C; and CO2 modified with 5 and 10 wt.% of ethanol or ethyl acetate. Total SFE yields ranged from 1.86 to 7.52 wt.%. The broken plus intact cells model (BICM), provided a good fitting of the SFE curves with 4.06 % error for total yield and 1.98–5.49 % for the individual yields of the said compounds. The BICM results revealed that the SFE is limited by intraparticle diffusion. Remarkable experimental and calculated selectivities were obtained for triterpenes (lupeol and β-amyrin), starting with a score of 1.7 and increasing along time to 10−100.



中文翻译:

葡萄叶超临界CO 2萃取:助溶剂和粒径对去除动力学和对目标化合物选择性的影响

葡萄的叶子含有潜在的生物活性化合物,即:长链脂族醇(例如1-己醇,1-辛醇和1-三十烷醇),α-生育酚,β-谷甾醇以及三萜类β-淀粉蛋白和羽扇豆酚。超临界流体萃取(SFE)运行是在实验室规模下使用以下方法测量的:压碎和磨碎的生物量;300 bar和40-80°C时的纯CO 2 ; 和CO 2用5和10重量%的乙醇或乙酸乙酯改性。SFE的总产率为1.86-7.52重量%。完整的残破细胞模型(BICM)提供了SFE曲线的良好拟合,该化合物的总产率误差为4.06%,单个化合物的产率为1.98–5.49%。BICM结果表明,SFE受颗粒内扩散限制。三萜类化合物(卢培醇和β-amyrin)的实验性和计算选择性显着提高,得分为1.7,并随时间增加至10-100。

更新日期:2020-06-25
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