Abstract

The demand for precursors obtained by biotechnological means is constantly growing in technical chemistry. The use of bioethanol is therefore of interest in the production of ethylene. In this work, Miscanthus sacchariflorus is used as a raw material for the production of bioethanol for the first time. The stage of the chemical treatment of miscanthus with 4 wt % nitric acid solution is successfully scaled-up under the conditions of pilot industrial production, and the products of nitric acid treatment (PNTs) are obtained with a yield of 37.4% and a 96.0% content of hydrolyzable components. It is shown that preliminary chemical treatment of miscanthus with nitric acid, regardless of its type, allows substrates with similar chemical compositions to be obtained. The process is scaled up for the first time in a fermenter with a capacity of 11 L (a scaling factor of 1 : 8) to combine the saccharification and fermentation of PNTs from miscanthus and raise the concentration of PNTs from 60.0 to 90.0 g/L. Commercially available enzyme preparations Cellolux-A and Bruzheim BGX are used for saccharification. Yeast strain VKPM Saccharomyces cerevisiae Y-1693 is used for fermentation. It is found that the concentration of bioethanol grows by 9.5 g/L upon raising the substrate concentration from 60.0 to 90.0 g/L. It is recommended that a substrate concentration of 90.0 g/L be used to scale up the process under trial industrial conditions. A basic scheme isproposed for producing bioethanol with a yield of bioethanol 202 L/t of miscanthus.

中文翻译：

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从芒草生产生物乙醇：初步扩大规模的经验

摘要

通过生物技术手段获得的前体的需求在技术化学中不断增长。因此，在乙烯生产中使用生物乙醇是令人感兴趣的。在这项工作中，芒草首次用作生产生物乙醇的原料。在中试工业生产的条件下，成功地扩大了用4 wt％硝酸溶液对虎钳进行化学处理的阶段，并获得了硝酸处理（PNTs）的产品，产率分别为37.4％和96.0％可水解成分的含量。结果表明，无论用哪种类型的硝酸，对花芒进行初步化学处理都可以得到化学成分相似的底物。该工艺首次在容量为11 L（比例因子为1：8）的发酵罐中进行规模放大，以结合糖化和发酵来自桔属植物的PNT并将其浓度从60.0 g / L提高至90.0 g / L 。可商购的酶制剂Cellolux-A和Bruzheim BGX用于糖化。酵母菌VKPM酿酒酵母Y-1693用于发酵。发现当底物浓度从60.0g / L提高到90.0g / L时，生物乙醇的浓度增长9.5g / L。建议在试验工业条件下使用90.0 g / L的底物浓度扩大工艺规模。提出了一种生产生物乙醇的基本方案，其生物乙醇的产量为桔梗202 L / t。