Due to the unique blue color and perennial growth habit, Muscari can be cultivated as a bedding plant, pot plant, or cut flower. Meanwhile, there is lack of information about genetic variations of Muscari neglectum Guss. ex Ten.; consequently, the diversity of nine populations (90 accessions) belonging to M. neglectum was evaluated by inter simple sequence repeat (ISSR) marker, start codon targeted (SCoT) marker and morphological marker. Ten ISSR primers used in this experiment amplified 142 reproducible bands with 137 (96.4%) polymorphism; and ten SCoT primers amplified 165 bands with 151 (91.12%) polymorphism. Both ISSR and SCoT analyses indicated a high level of genetic variation in the wild population of M. neglectum. Analysis of Molecular Variance (AMOVA) revealed a higher genetic diversity within the geographic group (0.69%) compared to the genetic variation among populations (0.31%). The gene flow between populations was low, while there was a greater gene flow between close areas. Morphological data divided nine populations into three clusters, while molecular data separated the populations into five groups, which were confirmed with structure analysis and discriminant analysis of principal component (DAPC) analysis. Our investigation showed that first, SCoT and ISSR markers are suitable for evaluation of genetic variation in Muscari genus, and there is appropriate genetic diversity among M. neglectum populations. Our results suggest some information to conserve the genetic resources and improve breeding program based on genetic data.

由于独特的蓝色和多年生的生长习性，穆斯卡里可以栽培为被褥植物，盆栽植物或切花。同时，缺乏关于穆斯卡里氏羊（Muscari neglectum Guss）遗传变异的信息。前十。因此，九个群体（90份）属于多样性M. neglectum通过帧间简单重复序列（ISSR）标记评估，起始密码子靶向（SCOT）标记和形态标记。本实验中使用的十种ISSR引物扩增了142个可重复的条带，具有137个（96.4％）多态性。10个SCoT引物扩增了165条带，具有151个（91.12％）多态性。ISSR和SCoT分析均表明，M。neglectum的野生种群中存在高水平的遗传变异。。分子差异分析（AMOVA）显示，与人群之间的遗传变异（0.31％）相比，地理区域内的遗传多样性更高（0.69％）。种群之间的基因流较低，而封闭区域之间的基因流较大。形态学数据将九个种群分为三类，而分子数据将种群分为五类，这通过结构分析和主成分判别分析（DAPC）分析得到了证实。我们的调查表明，第一，苏格兰人和ISSR分子标记适用于遗传变异的评价葡萄属，并有适当的间遗传多样性M. neglectum人口。我们的结果提出了一些信息，以保护遗传资源并根据遗传数据改进育种程序。