Flies of the genus Bradysia (Diptera, Sciaridae) are considered as major insect pests of greenhouse-grown horticultural crops. The ability of hooked trichomes of the French bean Phaseolus vulgaris to impale and entrap herbivorous insects thus resulting in insect reduced longevity, reproduction and larval mortality is well known. The present study investigates under laboratory conditions the ability of hooked trichomes of bean leaves to entrap fungus gnats Bradysia paupera, in order to estimate the reduction of their population. We characterized the entrapment mechanism of hooked trichomes towards B. paupera using cryo-scanning electron microscopy, and evaluated the silicon distribution in hooked trichomes with the energy dispersive X-ray microanalysis. We evaluated the trapping efficiency of hooked trichomes in fertilized and unfertilized bean plants towards B. paupera, in comparison with insects feeding on the plant leaf such as black bean aphid Aphis fabae and young stages of the southern green stinkbug Nezara viridula. For B. paupera, we recorded about 30% of entrapped insects in unfertilized plants. Considering the number of entrapped insects in relation to the leaf surface, the percentage of entrapped insect was higher in unfertilized than in fertilized plants having lower density of hooked trichomes. The presence of P. vulgaris plants in greenhouses could represent a useful method in integrated pest management to reduce Bradysia spp. population.

中文翻译：

---

菜豆（Fabaceae）植物叶片包埋迟缓（Didyera：Sciaridae）

布雷迪亚属（Diptera，Sciaridae）的苍蝇被认为是温室种植园艺作物的主要害虫。众所周知，菜豆菜豆的钩状毛状体刺穿和捕获草食性昆虫，从而导致昆虫的寿命，繁殖和幼虫死亡率降低的能力。本研究在实验室条件下研究了钩状的毛豆毛状线虫诱捕真菌贪食性短鞭毛虫的能力，以估计其种群减少。我们表征了钩状毛状体向B. paupera的诱捕机制使用低温扫描电子显微镜，并通过能量色散X射线显微分析评估钩状毛状体中的硅分布。我们评估了钩毛的捕集效率在受精和未受精的豆类植物对B. paupera，在与昆虫对植物叶片喂养如黑豆蚜虫比较蚜和南部绿色椿象的年轻阶段，稻绿蝽。对于枯草芽孢杆菌，我们记录了未受精植物中约30％的被捕获昆虫。考虑到与叶表面有关的被捕获昆虫的数量，未受精的被捕获昆虫的百分比高于钩状毛状体密度较低的受精植物。存在的温室中的寻常型百草枯植物可能代表了一种综合有害生物管理方法，以减少缓缓性葡萄球菌。人口。