The purpose of this study was to investigate the effects of zinc and melatonin administration on interleukin-6, lipid peroxidation parameters, and element metabolism in DMBA-induced breast cancer in female rats. A total of 42 recently weaned Wistar rats were divided into 5 groups as follows: control (group 1), DMBA control (group 2), DMBA + zinc (group 3), DMBA + melatonin (group 4), and DMBA + melatonin and zinc (group 5). Malondialdehyde (MDA) and glutathione (GSH) levels in breast tissue and blood samples were determined via spectrophotometric methods. In addition, iron, magnesium, zinc, and copper levels in serum samples were determined by atomic emission, and plasma interleukin-6 levels were determined by ELISA method. The highest tissue and plasma MDA and the lowest tissue and erythrocyte GSH levels found in the study were in group 2; the highest tissue and erythrocyte GSH levels and the lowest tissue and plasma MDA levels are in group 5 (P < 0.05). Iron, magnesium, and zinc levels of groups 3, 4, and 5 were higher than the DMBA group without administration (group 2), but the copper values were significantly lower (P < 0.05). The highest IL-6 levels were determined in group 2 while IL-6 levels in the DMBA group (G5) treated with combined melatonin and zinc were lower than all other breast cancer groups (P < 0.05). According to the findings obtained in this presented study, combined zinc and melatonin therapy can contribute to the prevention of tumor growth by improving the disruption in element metabolism and suppressing IL-6 levels and reducing tissue damage that causes the cancer.

这项研究的目的是调查锌和褪黑激素的施用对DMBA诱导的雌性大鼠白细胞介素6，脂质过氧化参数和元素代谢的影响。将总共​​42只最近断奶的Wistar大鼠分为5组，分别为：对照组（第1组），DMBA对照（第2组），DMBA +锌（第3组），DMBA +褪黑激素（第4组），DMBA +褪黑激素和锌（第5组）。通过分光光度法测定乳腺组织和血液样品中的丙二醛（MDA）和谷胱甘肽（GSH）水平。另外，通过原子发射法测定血清样品中的铁，镁，锌和铜含量，并通过ELISA法测定血浆白细胞介素6含量。在研究中发现，最高的组织和血浆MDA以及最低的组织和红细胞GSH水平在第2组。P  <0.05）。组3、4和5的铁，镁和锌水平高于未给药的DMBA组（组2），但铜值显着降低（P  <0.05）。在第2组中确定了最高的IL-6水平，而褪黑激素和锌联合治疗的DMBA组（G5）中的IL-6水平低于所有其他乳腺癌组（P  <0.05）。根据本研究中获得的发现，锌和褪黑激素联合疗法可通过改善元素代谢的破坏和抑制IL-6的水平并减少引起癌症的组织损伤，从而有助于预防肿瘤的生长。