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The Effect of Zinc and Melatonin Administration on Lipid Peroxidation, IL-6 Levels, and Element Metabolism in DMBA-Induced Breast Cancer in Rats

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Abstract

The purpose of this study was to investigate the effects of zinc and melatonin administration on interleukin-6, lipid peroxidation parameters, and element metabolism in DMBA-induced breast cancer in female rats. A total of 42 recently weaned Wistar rats were divided into 5 groups as follows: control (group 1), DMBA control (group 2), DMBA + zinc (group 3), DMBA + melatonin (group 4), and DMBA + melatonin and zinc (group 5). Malondialdehyde (MDA) and glutathione (GSH) levels in breast tissue and blood samples were determined via spectrophotometric methods. In addition, iron, magnesium, zinc, and copper levels in serum samples were determined by atomic emission, and plasma interleukin-6 levels were determined by ELISA method. The highest tissue and plasma MDA and the lowest tissue and erythrocyte GSH levels found in the study were in group 2; the highest tissue and erythrocyte GSH levels and the lowest tissue and plasma MDA levels are in group 5 (P < 0.05). Iron, magnesium, and zinc levels of groups 3, 4, and 5 were higher than the DMBA group without administration (group 2), but the copper values were significantly lower (P < 0.05). The highest IL-6 levels were determined in group 2 while IL-6 levels in the DMBA group (G5) treated with combined melatonin and zinc were lower than all other breast cancer groups (P < 0.05). According to the findings obtained in this presented study, combined zinc and melatonin therapy can contribute to the prevention of tumor growth by improving the disruption in element metabolism and suppressing IL-6 levels and reducing tissue damage that causes the cancer.

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Acknowledgments

This study was supported by the Scientific Research Projects Coordinatorship of Selcuk University (SUBAPK; project no. 16202035).

Data

Combined zinc and melatonin treatment may contribute to prevent tumor growth by improving impairment in element metabolism and suppressing IL-6 levels and increased tissue damage that causes acceleration of cancer development.

Funding

This study was supported by the Scientific Research Projects Coordinatorship of Selcuk University (SUBAPK; project no. 16202035).

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Authors and Affiliations

  1. Medical Facultuy, Department of Medical Biology, KTO Karatay University, Konya, Turkey

    Elif Gulbahce-Mutlu

  2. Department of Physiology, Faculty of Medicine, Selçuk University, Konya, Turkey

    Saltuk Bugra Baltaci, Rasim Mogulkoc & Abdulkerim Kasim Baltaci

  3. Department of Biochemistry, Faculty of Medicine, Selçuk University, Konya, Turkey

    Esma Menevse

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  1. Elif Gulbahce-Mutlu
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  2. Saltuk Bugra Baltaci
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  4. Rasim Mogulkoc
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  5. Abdulkerim Kasim Baltaci
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Correspondence to Abdulkerim Kasim Baltaci.

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The authors declare that they have no confict of interest.

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This study was performed on Wistar type adult male rats.

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Research meets ethical guidelines is a required field. The study protocol was approved by Selçuk University Experimental Medicine Research and Application Center Laboratory Animals Ethics Board (No: 2016-32).

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Gulbahce-Mutlu, E., Baltaci, S.B., Menevse, E. et al. The Effect of Zinc and Melatonin Administration on Lipid Peroxidation, IL-6 Levels, and Element Metabolism in DMBA-Induced Breast Cancer in Rats. Biol Trace Elem Res 199, 1044–1051 (2021). https://doi.org/10.1007/s12011-020-02238-0

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