Finding an effective alternative way to aid defense cells to fight Streptococcus mutans was the main goal of this study. The effect of plant extracts from Curcuma longa L. (turmeric), Rosmarinus officinalis L. (rosemary), and Thymus vulgaris L. (thyme) was evaluated on murine macrophages (RAW 264.7) infected by S. mutans. Minimum inhibitory concentration (MIC) of the extracts was determined. Macrophages were infected by S. mutans and treated with each extract. From the supernatants, it was measured nitric oxide (NO) level. Posteriorly, RAW 264.7 were lysed to expose living and phagocytosed bacteria. Cytotoxicity was checked by lysosomal activity analysis, using neutral red assay. Each extract helped RAW 264.7 to eliminate S. mutans during infection, as observed by a significant bacterial reduction. Significant cell viability was also found. Besides, an increased production of NO was verified using R. officinalis L. and T. vulgaris L. extracts. The evaluated extracts demonstrated an effective action to assist RAW 264.7 to fight S. mutans during infection.

中文翻译：

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Curcuma longa L.（姜黄）、Rosmarinus officinalis L.（迷迭香）和 Thymus vulgaris L.（百里香）提取物有助于鼠巨噬细胞 (RAW 264.7) 在体外感染期间对抗变形链球菌

寻找一种有效的替代方法来帮助防御细胞对抗变形链球菌是本研究的主要目标。评估了来自 Curcuma longa L.（姜黄）、Rosmarinus officinalis L.（迷迭香）和 Thymus vulgaris L.（百里香）的植物提取物对 S. mutans 感染的鼠巨噬细胞 (RAW 264.7) 的影响。确定提取物的最小抑菌浓度 (MIC)。巨噬细胞被变形链球菌感染并用每种提取物处理。从上清液中测量一氧化氮 (NO) 水平。之后，RAW 264.7 被裂解以暴露活的和被吞噬的细菌。细胞毒性通过溶酶体活性分析来检查，使用中性红测定。每种提取物都有助于 RAW 264.7 在感染期间消除变形链球菌，正如通过显着的细菌减少所观察到的那样。还发现了显着的细胞活力。此外，使用 R. officinalis L. 和 T. vulgaris L. 提取物证实了 NO 产量的增加。评估的提取物证明了在感染期间协助 RAW 264.7 对抗变形链球菌的有效作用。